| تعداد نشریات | 286 |
| تعداد نشریات سازمان | 84 |
| تعداد شماره‌ها | 3,016 |
| تعداد مقالات | 33,722 |
| تعداد مشاهده مقاله | 44,956,591 |
| تعداد دریافت فایل اصل مقاله | 45,764,042 |
بررسی چندشکلی ژن‌های بتالاکتوگلوبولین، پرولاکتین و DGAT1 در گاومیش خوزستان | ||
| علوم دامی | ||
| مقاله 9، دوره 29، شماره 111، شهریور 1395، صفحه 87-96 اصل مقاله (1.07 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/asj.2016.106927 | ||
| نویسندگان | ||
| بهاره طاهری دزفولی* 1؛ حمیدرضا سیدآبادی2؛ سیما ساور سفلی3 | ||
| 1عضوهیأت علمی، بخش تحقیقات علوم دامی، مرکزتحقیقات و آموزش کشاورزی و منابع طبیعی استان خوزستان، سازمان تحقیقات، آموزش و ترویج کشاورزی، اهواز | ||
| 2عضوهیأت علمی ، مؤسسه تحقیقات علوم دامی کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرج | ||
| 3عضو هیأت علمی، مؤسسه تحقیقات علوم دامی کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرج | ||
| چکیده | ||
| تولید و ترکیبات شیر جزء دسته صفات کمی و چندژنی می‌باشند وتحت تأثیر تعداد زیادی ژن قرار دارند. این تحقیق به منظور تعیین چندشکلی ژن‌های بتالاکتوگلوبولین، پرولاکتین و DGAT1، به عنوان ژن‌های کاندید مؤثر برصفات تولید شیر و ترکیبات آن،با استفاده از تکنیک PCR-RFLP درگاومیش‌های استان خوزستان صورت گرفت. از تعداد 200 رأس از گاومیش‌های استان خوزستان نمونه خون تهیه و استخراج DNA از نمونه‌ها به روش بهینه شده و تغییریافته استخراج نمکی صورت گرفت. بعد از انجام مراحل استخراج DNA، ژنوتیپ حیوانات با استفاده از روش PCR-RFLP تعیین گردید. برای تعیین ژنوتیپ حیوانات، قطعه 262 جفت باز از ناحیه اگزون و اینترون 4 ژن بتالاکتوگلوبولین، قطعه 411 جفت باز از اگزون 8 ژن DGAT1 و قطعه 156 جفت بازی از ناحیه اگزون 3 ژن پرولاکتین تکثیر و به ترتیب با استفاده از آنزیم‌های محدودالاثر HeaIII، CfrI و RsaI مورد هضم قرار گرفتند. محصولات هضم شده با استفاده از آنزیم، جهت تعیین ژنوتیپ بر روی آگارز 2% الکتروفورز گردیده و با اتیدیوم برماید رنگ‌آمیزی شدند. نتایج این تحقیق نشان داد که در گاومیش‌های مورد مطالعه آلل B در جایگاه بتالاکتوگلوبولین، آلل A در جایگاه پرولاکتین و آلل K در جایگاه DGAT1 تثبیت شده است و برای هیچ یک از جایگاه‌ها چندشکلی مشاهده نشد | ||
| کلیدواژه‌ها | ||
| پرولاکتین؛ چندشکلی؛ گاومیش؛ ژن DGAT1 | ||
| عنوان مقاله [English] | ||
| Investigation of β-Lactoglobulin, Prolactin and DGAT1 genes polymorphism in Khuzestani buffalo | ||
| نویسندگان [English] | ||
| B. Taheri Dezfoli1؛ H. R. Seyedabadi2؛ S. Savar Sofla3 | ||
| چکیده [English] | ||
| Milk production and its components are belong to quantitative and polygenic traits and affected by many genes. This study was conducted to determine the polymorphism in β-lactoglobulin, prolactin and DGAT1 genes of buffaloes in Khuzestan province using PCR-RFLP technique. Therefore, blood samples were collected from 200 buffaloes of Khuzestan province and then DNA samples were extracted using optimal and modified salting-out method. After DNA extraction, the genotype of the animals was determined by PCR-RFLP. For genotyping of animals, the 262 bp fragment of exon and intron 4 of the beta-lactoglobulin, the 411 bp fragment of exon 8 of the DGAT1 and the 156 bp fragment of exon 3 of the prolactin genes were amplified and then digested using restriction enzyme HeaIII, CfrI and RsaI, respectively. Then restricted PCR products electrophoresed on 2% agaros gel and stained in etediumbromide. Results of this study showed that the allele of B, the allele of A and the allele of K has been fixed in beta-lactoglobulin, prolactin and DGAT1 loci in buffalo population of Khuzestan, respectively and polymorphism was not seen for any of these loci. | ||
| کلیدواژه‌ها [English] | ||
| Prolactin, Polymorphism, Buffalo and DGAT1 gene | ||
| مراجع | ||
|
نشریه ترویجی (1387). پرورش گاومیش درخوزستان. کمیته انتشارات ترویج و توسعه کشاورزی خوزستان. ص ص: 20-23. Aggrey, S.E., Sabour, M.P., Lin, C.Y., Zadworny, D. and Kuhnlein, U. (1998). Analysis of the β-lactoglobulin locus using the granddaughter design in the Canadian Holstein population. Canadian Journal of Animal Science.76: 245-248. Alfonso, E., Roja, R., Herrera, J.G., Ortega, M.E., Lemus, C., Cortez, C., et al. (2012). Polymorphism of the prolactin gene (PRL) and its relationship with milk production in American Swiss cattle. African Journal of Biotechnology. 11(29):7338-7343. Alipanah, M., Kalashnikova, L. and Rodionov, G. (2007). Association of prolactin gene variants with milk production traits in Russian Red Pied cattle. Iranian Journal of Biotechnology. 5(3): 158-161. Bhattacaharya, T.K. and Gandhi, R.S. (1997). Marker assisted selection (MAS) and its application in dairy cattle. Indian Dairyman.49:39-45. Biradar, S.M., Unaune, K.P., Dodamani, S., Mhatre, P.S., Londhe, S.P., Pawar, V.D. et al. (2014). Study of prolactin gene polymorphism in Murrah buffalo. Boleckova, J., Matejickova, J., Stipkova, M., Kyselova, J. and Barton, L. (2012). The association of five polymorphisms with milk production traits in CzechFleckvieh cattle. Czech Journal of Animal Science. 57(2): 45–53. Bovenhuis, H. and Schrooten, C. (2002).Quantitative trait loci for milk production traits in dairy cattle. In: Proceedings of 7th World Congress of Genetics Applied to Livestock Production. France, Montpellier, pp: 7-9. Cases, S., Smith, S.J., Zheng, Y.W., Myers, H.M., Lear, S.R., Sande, E., Novak, S., Collins, C., Welch, C.B., Lusis, A.J., Erickson, S.K. and Farese, R.V. (1998). Identification of a gene encoding an acyl CoA: diacylglycerolacyltransferase, a key enzyme in triacylglycerol synthesis. In: Proceedings of the National Academy of Sciences, USA 95, pp: 13018–13023. Gautier, M., Capitan, A., Fritz, S., Eggen, A., Biochard, D. and Druet, T. (2007). Characterization of the DGAT1 K232A and variable number of tandem repeat polymorphisms in French dairy cattle. Journal of Dairy Science.90: 2980-2988. Gouda, E.M., Galal, M.K. and Wasfy, M.A. (2011). Phenotypes, Genotypes and Allele Frequencies of β-lactoglobulin in Egyptian Cattle and Buffalo. Journal of Agricultural Science. 3(4).203-210. Hill, J.P. (1993). The relationship between β-lactoglobulin phenotype and milk composition in New Zealand dairy cattle. Journal of Dairy Science.76: 281-286. Hill, J.P., Boland, M.J., Creamer, L.K., Anema, S.G., Otter, D.E., Paterson, G.R., Lowe, R., Motion, R.L. and Thresher, W.C. (1996). Effect of the bovine β-lactoglobulin phenotype on the properties of β-lactoglobulin, milk composition, and dairy products. In: Macromolecular Interactions in Food Technology (Eds Parris N, Kato A, Creamer LK, Pearce J). ACS symposium series, 650: 281–294. In: http://pubs.acs.org/doi/abs/10.1021/bk-1996-0650.ch022. Javanrouh, A., Banabazi, M.H., Esmaeilkhanian, S.,Amirinia, C., Seyedabadi, H.R. and Emrani, H. (2006). Optimization on salting out method for DNA extraction from animal and poultry blood cells. In: Proceedings of 57thAnnual Meeting of the European Association for Animal Production. Turkey, Antalya. Kaplan, S. and Boztepe, S. (2010).The determination of prolactin gene polymorphism using PCR-RFLP method within Indigenous Anatolian water buffalo and Brown Swiss. In: Proceedings of 2nd International Symposium on Sustainable Development, Sarajevo. Kaupe, B., Winter, A., Fries, R. and Erhardt, G. (2004). DGAT1 polymorphism in Bos indicus and Bos taurus cattle breeds. Journal of Dairy Research. 71:182-187. Kumari, A.R., Singh, K.M., Soni, K.J., Patel, R.K., Chauhan, J.B. and Sambasiva Rao, K.R.S. (2008).Genotyping of the polymorphism within exon 3 of prolactin gene in various dairy breeds by PCR RFLP. Archives Animal Breeding. 51(3): 298-299. Ladani, D.D., Pipalia, D.L., Brahmkshtri, B.P., Rank, D.N., Joshi, C.G., Vataliya, P.H. and Solanki, J.V. (2003). PCR-RFLP polymorphism at prolactin locus in buffaloes. Buffalo Journal. 2: 237-242. Lunden, A., Nilsson, M. and Janson, L. (1997). Marked effect of beta-Lactoglobulin polymorphism on the ratio of casein to total protein in milk. Journal of Dairy Science. 80: 2996-3005. Madhu, T., Ramesh, V. and Bishnu, M. (2006). DGAT1 and ABCG2 polymorphism in Indian cattle (Bosindicus) and buffalo (Bubalusbubalis) breeds. BioMed Central Veterinary Research. 2:32.1-6. Meignanalakshmi, A., Mahalinga, N.A. and Nachimuthu, K. (2001). Identification of genetic polymorphism of beta- lactoglobulin gene locus in Red Sindhi cows by PCR-RFLP Analysis. International Journal of Animal Science. 16:223-226. Meignanalakshmi, S. and Mahalinga Nainar, A. (2009). PCR-RFLP analysis of beta-lactoglobulin gene in Murrah buffaloes. Tamilnadu Journal of Veterinary & Animal Sciences. 5(5): 194-197. Mishra, B., Tantia, M.S., Bharani, S., Kumar, T. and Vijh, R.K. (2007).Characterization of the DGAT1 gene in the Indian buffalo (Bubalusbubalis). Journal of Genetics and Molecular Biology. 30(4): 1097-1100. Mitra, A., Schleec, P., Balakrishnanf, R. and Pirchner. (1995). Polymorphisms at growth-hormone and prolactin loci in Indian cattle and buffalo. Journal of Animal Breeding and Genetics.112:71-74. Nair, A.K., Sirothia, A.R., Patel, R.K., Soni, K.J., Singh, K.M., Sirothia, K.A. and Gawande, T.R. (2011). Characterization of κ-casein and β-lactoglobulin gene in Nagpuri buffaloes (Bubalus bubalis). Wayamba Journal of Animal Science. 153-157. Othman, O.E., Zayed, F.A., El-Gawead A.A. and M.R.A. El-Rahman. (2011). Genetic polymorphism of three genes associated with milk trait in Egyptian buffalo. Journal of Genetic Engineering and Biotechnology. 9(2): 97–102. Ozdil, F. and Ilhan, F. (2012). DGAT1-exon8 polymorphism in Anatolian buffalo. Livestock Science. 149(1): 83-87. Patel, R.K., Chauhan, J.B., Singh, K.M. and Soni K.J. (2007). Genotype and Allele frequencies of κ-casein and β-lactoglobulin in Indian River buffalo bulls (Bubalus bubalis). The Buffalo Bulletin. 26(3): 63-66. Rachagani, S., Gupta, I.D., Gupta, N. and Gupta, S.C. (2006). Genotyping of beta-lactoglobulin gene by PCR-RFLP in Sahiwal and Tharparkar cattle breeds. BioMed Central Genetic.7: 31-34. Ren, D.X., Miao, S.Y., Chen, Y.L., Zou, C.X., Liang, X.W. and Liu, J.X. (2011). Genotyping of the κ -casein and β-lactoglobulin genes in Chinese Holstein, jersey and water buffalo by PCR-RFLP. Journal of Genetic Science. 90(1): e1-e5. Sabour, M.P., Lin, C.Y. and Keough, A. (1993). Effects of selection practiced on the frequencies of κ -casein and β -Lactoglobolin genotypes in Canadian artificial in insemination bulls. Journal of Dairy Science. 76:174-180. Shi, D.S., Wang, J., Yang, Y., Lu, F.H., Li, X.P. and Liu, Q.Y. (2012). DGAT1, GH, GHR, PRL and PRLR polymorphism in water buffalo (Bubalus bubalis). Reproduction Domestic Animals. 47(2):328-334. Spelman, R.J., Ford, C.A., McElhinney, P., Gregory, G.C. and Snell, R.G. (2002). Characterization of the DGATl gene in the New Zealand dairy population. Journal of Dairy Science.85:3514-3517. Tahira, I., Mahmood, A., Saqlain, M., Hanif, N.Q. and Kaukab Raja, G. (2014). Study of β-Lactoglobulin milk protein variants in buffalo. Pakistan Journal of Zoology. 46(2):549-552. Tantia, M.S., Vijh, R.K., Mishra, B.P., Mishra, B., Kumar, S.T.B. and Sodhi, M. (2006). DGAT1 and ABCG2 polymorphism in Indian cattle (Bos indicus) and buffalo (Bubalus bubalis) breeds.BioMed Central Veterinary Research. 2:32. Tsiaras, A.M., Bargouli, G.G., Banos, G. and Boscos, C.M. (2005). Effect of kappa Casein and beta Lactoglobulin loci on milk production traits and reproductive performance. Journal of Dairy Science.88:327-334. Van der Berg, G., Escher, J.T.M., Konning, P.J. and Bovenhuis, H. (1992). Genetic polymorphism of κ -casein and β -lactoglobulin in relation to milk composition and processing. Netherlands Milk and Dairy Journal.46: 145-168. Vohra, V., Bhattacharya, T.K., Dayal, S., Kumar, P. and Sharma, A. (2006). Genetic variants of beta-lactoglobulin gene and its association with milk composition traits in riverine buffalo. Journal of Dairy Research. 73(4):499-503. Weiss, B., Davidkova, G. and Zhou, L.W. (1999). Antisense RNA gene therapy for studying and modulating biological processes. Cellular and Molecular Life Sciences.55: 334-358. Weller, J.I., Golik, M., Seroussi, E., Ezra,E. and Ron, M. (2003). Population-wide analysis of a QTL affecting milk-fat production in the Israeli Holstein population. Journal of Dairy Science.86: 2219-2227. Yuan, J., Zhou, J., Deng, X., Hu, X. and Li, N. (2007). Molecular cloning and single nucleotide polymorphism detection of buffalo DGAT1 gene. Biochemical Genetics. 45(7-8):611-621. | ||
|
آمار تعداد مشاهده مقاله: 732 تعداد دریافت فایل اصل مقاله: 691 |
||