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بررسی چندشکلی ژن‌های بتالاکتوگلوبولین، پرولاکتین و DGAT1 در گاومیش خوزستان | ||
| علوم دامی | ||
| مقاله 9، دوره 29، شماره 111، شهریور 1395، صفحه 87-96 اصل مقاله (1.07 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/asj.2016.106927 | ||
| نویسندگان | ||
| بهاره طاهری دزفولی* 1؛ حمیدرضا سیدآبادی2؛ سیما ساور سفلی3 | ||
| 1عضوهیأت علمی، بخش تحقیقات علوم دامی، مرکزتحقیقات و آموزش کشاورزی و منابع طبیعی استان خوزستان، سازمان تحقیقات، آموزش و ترویج کشاورزی، اهواز | ||
| 2عضوهیأت علمی ، مؤسسه تحقیقات علوم دامی کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرج | ||
| 3عضو هیأت علمی، مؤسسه تحقیقات علوم دامی کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرج | ||
| چکیده | ||
| تولید و ترکیبات شیر جزء دسته صفات کمی و چندژنی می‌باشند وتحت تأثیر تعداد زیادی ژن قرار دارند. این تحقیق به منظور تعیین چندشکلی ژن‌های بتالاکتوگلوبولین، پرولاکتین و DGAT1، به عنوان ژن‌های کاندید مؤثر برصفات تولید شیر و ترکیبات آن،با استفاده از تکنیک PCR-RFLP درگاومیش‌های استان خوزستان صورت گرفت. از تعداد 200 رأس از گاومیش‌های استان خوزستان نمونه خون تهیه و استخراج DNA از نمونه‌ها به روش بهینه شده و تغییریافته استخراج نمکی صورت گرفت. بعد از انجام مراحل استخراج DNA، ژنوتیپ حیوانات با استفاده از روش PCR-RFLP تعیین گردید. برای تعیین ژنوتیپ حیوانات، قطعه 262 جفت باز از ناحیه اگزون و اینترون 4 ژن بتالاکتوگلوبولین، قطعه 411 جفت باز از اگزون 8 ژن DGAT1 و قطعه 156 جفت بازی از ناحیه اگزون 3 ژن پرولاکتین تکثیر و به ترتیب با استفاده از آنزیم‌های محدودالاثر HeaIII، CfrI و RsaI مورد هضم قرار گرفتند. محصولات هضم شده با استفاده از آنزیم، جهت تعیین ژنوتیپ بر روی آگارز 2% الکتروفورز گردیده و با اتیدیوم برماید رنگ‌آمیزی شدند. نتایج این تحقیق نشان داد که در گاومیش‌های مورد مطالعه آلل B در جایگاه بتالاکتوگلوبولین، آلل A در جایگاه پرولاکتین و آلل K در جایگاه DGAT1 تثبیت شده است و برای هیچ یک از جایگاه‌ها چندشکلی مشاهده نشد | ||
| کلیدواژه‌ها | ||
| پرولاکتین؛ چندشکلی؛ گاومیش؛ ژن DGAT1 | ||
| عنوان مقاله [English] | ||
| Investigation of β-Lactoglobulin, Prolactin and DGAT1 genes polymorphism in Khuzestani buffalo | ||
| نویسندگان [English] | ||
| B. Taheri Dezfoli1؛ H. R. Seyedabadi2؛ S. Savar Sofla3 | ||
| چکیده [English] | ||
| Milk production and its components are belong to quantitative and polygenic traits and affected by many genes. This study was conducted to determine the polymorphism in β-lactoglobulin, prolactin and DGAT1 genes of buffaloes in Khuzestan province using PCR-RFLP technique. Therefore, blood samples were collected from 200 buffaloes of Khuzestan province and then DNA samples were extracted using optimal and modified salting-out method. After DNA extraction, the genotype of the animals was determined by PCR-RFLP. For genotyping of animals, the 262 bp fragment of exon and intron 4 of the beta-lactoglobulin, the 411 bp fragment of exon 8 of the DGAT1 and the 156 bp fragment of exon 3 of the prolactin genes were amplified and then digested using restriction enzyme HeaIII, CfrI and RsaI, respectively. Then restricted PCR products electrophoresed on 2% agaros gel and stained in etediumbromide. Results of this study showed that the allele of B, the allele of A and the allele of K has been fixed in beta-lactoglobulin, prolactin and DGAT1 loci in buffalo population of Khuzestan, respectively and polymorphism was not seen for any of these loci. | ||
| کلیدواژه‌ها [English] | ||
| Prolactin, Polymorphism, Buffalo and DGAT1 gene | ||
| مراجع | ||
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