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بررسی اثرات مقدار اینوکلوم و سرعت هوادهی بر تولید بیوماس و اسید شیکوریک در کشت ریشه‌های مویین گیاه سرخارگل (Echinacea purpurea L.) در بیوراکتور ستون حباب‌دار | ||
| تحقیقات گیاهان دارویی و معطر ایران | ||
| مقاله 2، دوره 32، شماره 3 - شماره پیاپی 77، شهریور 1395، صفحه 377-388 اصل مقاله (279.39 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijmapr.2016.106821 | ||
| نویسندگان | ||
| فتح‌الله احمدی1؛ احمد معینی* 2؛ سجاد رشیدی منفرد3 | ||
| 1فارغ‌التحصیل کارشناسی ارشد بیوتکنولوژی کشاورزی، دانشکده کشاورزی، دانشگاه تربیت مدرس، تهران، ایران | ||
| 2دانشیار، گروه اصلاح نباتات و بیوتکنولوژی، دانشکده کشاورزی، دانشگاه تربیت مدرس، تهران، ایران | ||
| 3استادیار، گروه اصلاح نباتات و بیوتکنولوژی، دانشکده کشاورزی، دانشگاه تربیت مدرس، تهران، ایران | ||
| چکیده | ||
| در این پژوهش، ریشه‌های مویین گیاه سرخارگل (Echinacea purpurea L.) در بیوراکتور ستون حباب‌دار 1000 میلی‌لیتری حاوی 500 میلی‌لیتر محیط کشت WPM مایع کشت و تأثیر مقادیر اینوکلوم (3، 6 و gl-19) و سرعت‌های هوادهی (1/0، 2/0 و vvm4/0) روی میزان تولید بیوماس و اسید شیکوریک بررسی شدند. صفات وزن تر، وزن خشک و مقدار اسید شیکوریک ریشه‌های مویین بعد از 30 روز اندازه‌گیری شدند. نتایج نشان داد که بیشترین مقدار بیوماس (g l-14/16 وزن تر و g l-1084/2 وزن خشک) و اسید شیکوریک (mg g-1 DW74/16) در مقدار اینوکلوم g l-1 6 تولید شده است. نتایج حاصل از بررسی اثر سرعت هوادهی بر تولید بیوماس و اسید شیکوریک نیز نشان داد که بیشترین میزان تولید وزن تر ( g l-14/15) و وزن خشک ( g l-1467/2) در سرعت هوادهی vvm 4/0 و بیشترین میزان تولید اسید شیکوریک (mg g-1 DW 74/12) در سرعت هوادهی vvm 2/0 بدست آمده است. به‌طور کلی مقدار اینوکلوم و سرعت هوادهی نقش مهمی در میزان رشد و نمو ریشه‌های مویین در بیوراکتور ستون حباب‌دار داشتند و برای بهینه کردن تولید بیوماس و متابولیت ثانویه باید به این عوامل توجه کرد. | ||
| کلیدواژه‌ها | ||
| سرخارگل (Echinacea purpurea L.)؛ متابولیت ثانویه؛ گیاه دارویی؛ کشت درون شیشه‌ای | ||
| عنوان مقاله [English] | ||
| Effects of inoculum density and aeration rate on biomass and cichoric acid production in Echinacea purpurea L. hairy roots in bubble column bioreactor | ||
| نویسندگان [English] | ||
| F. Ahmadi1؛ A. Moieni2؛ S. Rashidi Monfared3 | ||
| 1MSc., Department of Plant Breeding and Biotechnology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran | ||
| 2Department of Plant Breeding and Biotechnology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran | ||
| 3Department of Plant Breeding and Biotechnology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran | ||
| چکیده [English] | ||
| In this research, Echinacea purpurea L. hairy roots were cultured in 1000 ml bubble column bioreactor containing 500 ml WPM liquid medium and the effects of inoculum densities (3, 6 and 9g l-1) and aeration rates (0.1, 0.2 and 0.4vvm) on biomass and cichoric acid production were investigated. Fresh and dry weights and also the cichoric acid content in hairy roots were measured after 30 days. The results showed that the highest amount of biomass (16.4g l-1 fresh weight and 2.084g l-1 dry weight) and cichoric acid (16.74mg g-1 DW) were produced in the inoculum density of 6 gl-1. The investigation of aeration rates on biomass and cichoric acid production showed that the maximum fresh weight (15.4g l-1) and dry weight (2.467g l-1) were obtained in aeration rate of 0.4vvm and the maximum cichoric acid content (12.74mg g-1 DW) was obtained in aeration rate of 0.2vvm. Overall, the inoculum density and aeration rate had considerable effects on the hairy root growth and development in bubble column bioreactor and they should be optimized for obtaining the highest hairy root biomass and secondary metabolites. | ||
| کلیدواژه‌ها [English] | ||
| Echinacea purpurea L, secondary metabolite, medicinal plant, in vito culture | ||
| مراجع | ||
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