| تعداد نشریات | 285 |
| تعداد نشریات سازمان | 83 |
| تعداد شمارهها | 3,094 |
| تعداد مقالات | 34,531 |
| تعداد مشاهده مقاله | 49,373,544 |
| تعداد دریافت فایل اصل مقاله | 79,820,487 |
تأثیر نگهداری طولانیمدت اسانس ترخون (Artemisia dracunculus L.) بر ظرفیت آنتیاکسیدانی و LD50 آن | ||
| تحقیقات گیاهان دارویی و معطر ایران | ||
| مقاله 7، دوره 32، شماره 1، فروردین 1395، صفحه 60-70 اصل مقاله (271.56 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijmapr.2016.106137 | ||
| نویسندگان | ||
| قادر جلیلزاده امین* 1؛ نجمه شمشیری2؛ جواد علیاکبرلو3 | ||
| 1استادیار، دانشکده دامپزشکی، دانشگاه ارومیه، ایران | ||
| 2دانشآموخته دکترای عمومی، دانشکده دامپزشکی، دانشگاه ارومیه، ایران | ||
| 3استادیار، بهداشت مواد غذایی، دانشکده دامپزشکی، دانشگاه ارومیه، ایران | ||
| چکیده | ||
| داروهای گیاهی بهدلیل طبیعی بودن ترکیبهای سالمی هستند ولی عوارض سوئی به دنبال استفاده بیرویه از آنها اتفاق میافتد. هدف این پژوهش ارزیابی سمّیت حاد و فعالیت آنتیاکسیدانی اسانس ترخون (Artemisia dracunculus L.) میباشد. اسانس ترخون توسط دستگاه کلونجر با روش تقطیر با آب استخراج شد. تمام پروتکلهای آزمایش برای اسانس تازه و اسانس کهنه که به مدت سه ماه در شرایط آزمایشگاهی نگهداری شده بود، انجام گردید. میزان LD50 براساس روش لورک و با روش آنالیز احتمال و Maximum Likelihood با استفاده از نرمافزار MINITAB تعیین شد. برای بررسی مسمومیت حاد و تغییرات رفتاری ایجاد شده از دوزهای (mg/kg) 10، 100، 1000،1600،2900 و 5000 اسانس (به روش خوراکی و تزریق داخل صفاقی) استفاده گردید. فعالیت آنتیاکسیدانی اسانس دوزهای مختلف (mg/ml32-1)، با بهرهگیری از دو روش DPPH و ABTS ارزیابی شد. مقدار LD50 برای اسانس ترخون تازه و کهنه در روش خوراکی بهترتیب بیشتر از g/kg5 و mg/kg33/3801 وزن بدن بود. با تزریق داخل صفاقی مقدار LD50 برای اسانس تازه و اسانس کهنه بهترتیب 06/4810 و 33/3013 میلیگرم به ازای هر کیلوگرم وزن بدن تعیین شد. اسانس تازه فعالیت آنتیاکسیدانی قویتری را در مقایسه با اسانس کهنه در هر دو روش ارزیابی نشان داد. یافتههای اولیه این تحقیق نشان میدهد که اسانس این گیاه غیر سمّی بوده و دارای ترکیبهای آنتیاکسیدانتی است که مؤید استفاده از این گیاه در طب سنتی میباشد. | ||
| کلیدواژهها | ||
| سمّیت حاد؛ زمان نگهداری؛ ترخون (Artemisia dracunculus L.)؛ آنتیاکسیدان | ||
| عنوان مقاله [English] | ||
| Effect of long-term storage of Artemisia dracunculus L. essential oil on antioxidant capacity and LD50 value | ||
| نویسندگان [English] | ||
| Gh. Jalilzadeh-Amin1؛ N. Shamshiri2؛ J. Aliakbarlu3 | ||
| 1Department of Internal Medicine and Clinical Pathology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran | ||
| 2Faculty of Veterinary Medicine, Urmia University, Urmia, Iran | ||
| 3Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, University of Urmia, Urmia, Iran | ||
| چکیده [English] | ||
| Herbal medicines are natural and safe; however, side effects can occur following excessive use of them. This research was aimed to investigate the acute toxicity and antioxidant activity of the essential oil of Artemisia dracunculus L. The essential oil of A. dracunculus (EOAD) was extracted by Clevenger apparatus using hydrodistillation. Two types of essential oil were studied including fresh and old oil (kept in laboratory conditions for three months). All protocols were tested for both fresh and old oil. The LD50 was calculated based on Lorke’s method using Probit Analysis and maximum Likelihood method with MINITAB software. To induce acute toxicity, appropriate concentrations of essential oil (10, 100, 1000, 1600, 2900 and 5000mg/kg of EOAD) were given orally and intraperitoneally. The antioxidant activity of EOAD (1-32mg/ml) was measured by two methods: DPPH and ABTS assays. The oral LD50 values were greater than 5000 mg/kg and 3801.33mg/kg for fresh and old EO, respectively. When fresh and old EO administered intraperitoneally, the LD50 values were calculated to be 4810.06mg/kg and 3013.33mg/kg, respectively. The fresh essential oil showed higher antioxidant activity as compared with the old essential oil in both DPPH and ABTS methods. Our results clearly indicate that the essential oil of this plant is non-toxic and contains antioxidant compounds that support the use of tarragon in traditional medicine. | ||
| کلیدواژهها [English] | ||
| Acute toxicity, Storage, Artemisia dracunculus L, Antioxidant | ||
| مراجع | ||
|
- نوری قنبلانی، ق.، فتحی، س.ع.ا. و برمکی، م.، 1389. تأثیر برخی از اسانسهای گیاهی روی رفتارهای تخمگذاری و تغذیهای سوسک کلرادوی سیبزمینی Leptinotarsa decemlineata Say (Col.: Chrysomelidae). گیاهپزشکی (مجله علوم کشاورزی)، 23(2): 9-1. - Abdalla, A.E. and Roozen, J.P., 1999. Effect of plant extracts on the oxidative stability of sunflower oil and emulsion. Food Chemistry, 64(3): 323-329. - Ayoughi, F.M., Barzegar, M., Sahari, M.A. and Naghdibadi, H., 2011. Chemical compositions of essential oils of Artemisia dracunculus L. and endemic Matricaria chamomilla L. and an evaluation of their antioxidative effects. Journal of Agricultural Science and Technology, 13: 79-88. - Boivin, J. and Schmidt, L., 2009. Use of complementary and alternative medicines associated with a 30% lower onging pregnancy, live birth rate during 12 months of fertility treatment. Human Reproduction, 24(7): 1626-1631. - Deans, S.G. and Simpson, E.J.M., 2002. Artemisia dracunculus industrial profiles: 91-97. In: Wright, C.W., (Ed.). Artemisia: Medicinal and Aromatic Plants. Taylor & Francis, London, 358p. - Elvin-Lewis, M., 2001. Should we be concerned about herbal remedies. Journal of Ethnopharmacology, 75: 141-167. - Erel, B.S., Reznicek, G., Şenol, S.G., Se, N., Yavasogulu, U.K., Konyalioglu, S. and Zeybek, A.U., 2010. Antimicrobial and antioxidant properties of Artemisia L. species from western Anatolia. Turkish Journal of Biology, 36: 75-84. - Espin, J.C., Soler-Rivas, C. and Wichers, H.J., 2000. Characterisation of the total free radical scavenger capacity of vegetable oils and oil fractions using 2.2-diphenyl-1-picrylhydrazyl radical. Journal of Agricultural and Food Chemistry, 48(3): 648-656. - Gordon, W.P., Forte, A.J., Mac-Murtry, R.J., Gal, J. and Nelson, S.D., 1982. Hepatotoxicity and pulmonary toxicity of pennyroyal oil and its constituent terpenes in the mouse. Toxicology and Applied Pharmacology, 65(3): 413-424. - Jalilzadeh-Amin, G., Maham, M., Dalir-Naghadeh, B. and Kheiri, F., 2012. In vitro effects of Artemisia dracunculus essential oil on ruminal and abomasal smooth muscle in sheep. Comparative Clinical Pathology, 21: 673-680. - Jang, I.C., Jo, E.K., Bae, M.S., Lee, H.J., Jeon, G.I., Park, E., Yuk, H.G., Ahn, G.H. and Lee, S.C., 2010. Antioxidant and antigenotoxic activities of different parts of persimmon (Diospyros kaki cv. fuyu) fruit. Journal of Medicinal Plant Research, 4: 155-160. - Jothy, S.L., Zakaria, Z., Chen, Y., Lau, Y.L., Latha, L.Y. and Sasidharan, S., 2011. Acute oral toxicity of methanolic seed extract of Cassia fistula in mice. Molecules, 16(6): 5268-5282. - Kordali, S., Kotan, R., Mavi, A., Cakir, A., Ala, A. and Yildirim, A., 2005. Determination of the chemical composition and antioxidant activity of the essential oil of Artemisia dracunculus and of the antifungal and antibacterial activities of Turkish Artemisia absinthium, A. dracunculus, Artemisia santonicum and Artemisia spicigera essential oils. Journal of Agricultural and Food Chemistry, 53(24): 9452-9458. - Loomis, T.A. and Hayes, A.W., 1996. Loomis’s Essentials of Toxicology. Academic Press, California, 282p. - Lopes-Lutz, D., Alviano, D.S., Alviano, C.S. and Kolodziejczyk, P.P., 2008. Screening of chemical composition, antimicrobial and antioxidant activities of Artemisia essential oils. Phytochemistry, 69(8): 1732-1738. - Lorke, D., 1983. A new approach to practical acute toxicity testing. Archive of Toxicology, 54(4): 275-287. - Maham, M., Moslemzadeh, H. and Jalilzadeh-Amin, G., 2014. Antinociceptive effect of the essential oil of tarragon (Artemisia dracunculus). Pharmaceutical Biology, 52(2): 208-212. - Mehrotra, S., Rawat, A.K.S. and Shome, U., 1993. Antimicrobial activity of the essential oils of some Indian Artemisia species. Fitoterapia, 64: 65-68. - Miller, N.J., Rice-Evans, C.A., Davies, M.J., Gopinathan, V. and Milner, A., 1993. A novel method for measuring antioxidant capacity and its application tomonitoring the antioxidant status in premature neonates. Clinical Science, 84: 407-412. - Millet, Y., Jouglard, J., Steinmetz, M.D., Tognetti, P., Joanny, P. and Arditti, J., 1981. Toxicity of some essential plant oils-clinical and experimental study. Clinical Toxicology, 18(12): 1485-1498. - Misharina, T.A., Polshkov, A.N., Ruchkina, E.L. and Medvedeva, I.B. 2003. Changes in the composition of the essential oil of marjoram during storage. Applied Biochemistry and Microbiology, 39(3): 353-358. - Misharina, T.A., 2001. Effect of conditions and duration of storage on composition of essential oil from coriander seeds. Prikladnaia Biokhimiia Mikrobiologiia, 37(6): 726-732. - Mukinda, J.T. and Syce, J.A., 2007. Acute and chronic toxicity of the aqueous extract of Artemisia afra in rodents. Journal of Ethnopharmacology, 112: 138-144. - Raeisi, M., Tajik, H., Razavi Roohani, S.M., Maham, M., Moradi, M., Hajimohammadi, B., Naghili, H., Hashemi, M. and Mehdizadeh, T., 2012. Essential oil of tarragon (Artemisia dracunculus) antibacterial activity on Staphylococcus aureus and Escherichia coli in culture media and Iranian white cheese. Iranian Journal of Microbiology, 4(1): 30-34. - Ribnicky, D.M., Poulev, A., O'Neal, J., Wnorowski, G., Malek, D.E., Jäger, R. and Raskin, I., 2004. Toxicological evaluation of the ethanolic extract of Artemisia dracunculus L. for use as a dietary supplement and in functional foods. Food and Chemical Toxicology, 42(4): 585-598. - Ruberto, G. and Baratta, M.M.T., 2000. Antioxidant activity of selected essential oil components in two lipid model systems. Food Chemistry, 69(2): - Sharafati Chaleshtori, R., Rokni, N., Razavilar, V. and Rafieian, M., 2013. The evaluation of the antibacterial and antioxidant activity of tarragon (Artemisia dracunculus L.) essential oil and its chemical composition. Jundishapur Journal of Microbiology, 6(9): e7877. - Sharma, N. and Tripathi, A., 2008. Effects of Citrus sinensis (L.) Osbeck epicarp essential oil on growth and morphogenesis of Aspergillus niger (L.) Van Tieghem. Microbiological Research, 163(3): 337-344. - Shi, F., Jia, X., Zhao, C. and Chen, Y., 2010. Antioxidant activities of various extracts from Artemisia selengensis Turcz (LuHao). Molecules, 15(7): 4934-4946. - Tariku, Y., Hymete, A., Hailu, A. and Rohloff, J., 2010. In vitro evaluation of antileishmanial activity and toxicity of essential oils of Artemisia absinthium and Echinops kebericho. Chemistry and Biodiversity; 8(4): 614-623. - Tomlinson, T.R., 1998. Medicinal Plants: Their Role in Health and Biodiversity. University of Pennsylvania Press, Philadelphia, 221p. - Vigan, M., 2010. Essential oils: renewal of interest and toxicity. European Journal of Dermatology, 20(6): 685-692. | ||
|
آمار تعداد مشاهده مقاله: 1,247 تعداد دریافت فایل اصل مقاله: 932 |
||