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ساختار ژنتیکی جمعیتهای Fusarium verticillioides عامل پوسیدگی ریشه و طوقه برنج در استان ایلام با استفاده از نشانگرهای ریزماهواره‌ای (مقاله‌ی انگلیسی) | ||
| آفات و بیماری‌های گیاهی | ||
| مقاله 20، دوره 83، شماره 2 - شماره پیاپی 101، اسفند 1394، صفحه 13-22 اصل مقاله (240 K) | ||
| نوع مقاله: بیماری‌شناسی گیاهی | ||
| شناسه دیجیتال (DOI): 10.22092/jaep.2016.106126 | ||
| نویسندگان | ||
| خشنود نوراللهی* 1؛ زینب حقی2 | ||
| 1استادیار بیماری‌شناسی گیاهی گروه گیاهپزشکی دانشگاه ایلام | ||
| 2دانشجوی سابق کارشناسی ارشد بیماری‌شناسی گیاهی گروه گیاهپزشکی دانشگاه ایلام | ||
| چکیده | ||
| مطالعه ساختار ژنتیکی جمعیتهای Fusarium verticillioides اطلاعات مختلفی از مدیریت بیماری پوسیدگی ریشه در مزارع برنج را فراهم میکند. نشانگرهای SSR برای تعیین ساختار ژنتیکی و تخمین تنوع ژنتیکی در 39 جدایهF. verticillioides از پنج منطقه مختلف از جمله: تنگه قیر، لومار، شیروان، سرابله و دره شهر در استان ایلام استفاده شد. میانگین آللها در هرمکان ژنی 5/2 آلل بود و و تعداد آللها در جمعیتها از جمله جمعیت لومار با تعداد 13 آلل بیشترین آلل و جمعیتهای تنگه قیر و دره شهر با نه آلل بهعنوان کمترین تعداد آلل را دارا بودند. تعداد آللهای مشاهده شده(Na) و تعداد آللهای مؤثر (Ne) در لومار (1/276= Ne : 1= Na) نسبت به دیگر جمعیتها بیشتر بودند. تنوع ژنتیکی (H) و شاخص شانون (I) در لومار بالا بود) 0/167 =He 0/254 = I (اما مقدار آنها در دره شهر کمتر بود) 0/141 =He 0/204 = I). کمترین فاصله ژنتیکی بین تنگه قیر و لومار و بعد شیروان مشاهده شد و بیشترین فاصله ژنتیکی بین دره شهر و تنگ قیر (0/018) وجود داشت. تنوع ژنتیکی کل(Ht) و تنوع ژنی بین زیر جمعیتها (Hs) 0/125 و 0/116 بهترتیب تخمین زده شد. تنوع ژنتیکی مؤثر در تمایز بین جمعیتها (Gst) 0/072 بود و میزان جریان ژنی(Nm) 6/368 بود. تجزیه کلاستر براساس UPGMA نشان داد که بین تنگه قیر و لومار و سپس شیروان کمترین فاصله ژنتیکی وجود دارد و دندروگرام فاصله ژنتیکی بالایی بین دره شهر و چهار جمعیت دیگر را نشان میدهد. | ||
| کلیدواژه‌ها | ||
| برنج؛ ساختار ژنتیکی؛ Fusarium verticillioides؛ ریزماهواره | ||
| عنوان مقاله [English] | ||
| Population genetic structure of Fusarium verticillioides causal agent of rice crown and root rot in Ilam province using SSR Marker (Article in English) | ||
| نویسندگان [English] | ||
| KH. NOUROLLAHI1؛ Z. HAGHI2 | ||
| چکیده [English] | ||
| Study on the genetic structure of Fusarium verticillioides populations, provides different levels of information in management of root rot disease in rice farms.Simple Sequence repeat (SSR) markers were used to determine genetic structure and genetic diversity in 39 F. verticillioides isolates from five different regions in Ilam province, such as: Tangehghir, Lomar, Shirvan, Sarableh and Darehshahr. Average number of alleles in populations were 10.6, the number of alleles in populations varied from 9 allele in Tangehghir and Darehshahr as the lowest to 13 allels in Lomaras the highest. Observed number of alleles (Na) and effective number of alleles (Ne) were higher in Lomar (Na = 1; Ne = 1.276) compared to other populations. The genetic diversity (H)and Shannon's Information index (I) were also higher in Lomar (H = 0.167; I = 0.254) but lower values were estimated for Darehshahr (He = 0.141; I = 0.204). The lowest genetic distance was found between Tangehghir and Lowmar (0.00) and then Shirvan, while the highest genetic distance was revealed between Darehshahr and Tangehghir (0.018). Total gene diversity (Ht) and gene diversities between subpopulations (Hs) were estimated 0.125 and 0.116, respectively. Gene diversity attributable to differentiation among populations (Gst) was 0.072, while gene flow (Nm) was 6.368. Cluster analysis based on UPGMA showed the lowest genetic distance between Tangehghir and Lomar and then between Shirvan. The dendrogram indicated a high genetic distance between Darehshahr and four remaining populations.Results from this study will be useful in developing necessary control methods for Rice crown and root rot disease. | ||
| کلیدواژه‌ها [English] | ||
| Fusarium verticillioides, Genetic structure, rice, SSR | ||
| مراجع | ||
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