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Marcellino, W.L., El Hussein, A.M., Salih, D.A., Berkvens, D., Geysen, D.. (1394). Molecular characterization of Theileria parva parasites from South Sudan using the PCR-RFLP approach on antigen genes. سامانه مدیریت نشریات علمی, 70(1), 13-20. doi: 10.7508/ari.2015.01.003
W.L. Marcellino; A.M. El Hussein; D.A. Salih; D. Berkvens; D. Geysen. "Molecular characterization of Theileria parva parasites from South Sudan using the PCR-RFLP approach on antigen genes". سامانه مدیریت نشریات علمی, 70, 1, 1394, 13-20. doi: 10.7508/ari.2015.01.003
Marcellino, W.L., El Hussein, A.M., Salih, D.A., Berkvens, D., Geysen, D.. (1394). 'Molecular characterization of Theileria parva parasites from South Sudan using the PCR-RFLP approach on antigen genes', سامانه مدیریت نشریات علمی, 70(1), pp. 13-20. doi: 10.7508/ari.2015.01.003
Marcellino, W.L., El Hussein, A.M., Salih, D.A., Berkvens, D., Geysen, D.. Molecular characterization of Theileria parva parasites from South Sudan using the PCR-RFLP approach on antigen genes. سامانه مدیریت نشریات علمی, 1394; 70(1): 13-20. doi: 10.7508/ari.2015.01.003

Molecular characterization of Theileria parva parasites from South Sudan using the PCR-RFLP approach on antigen genes

Archives of Razi Institute
مقاله 3، دوره 70، شماره 1، تیر 2015، صفحه 13-20    اصل مقاله (1.18 M)
شناسه دیجیتال (DOI): 10.7508/ari.2015.01.003
نویسندگان
W.L. Marcellino؛ A.M. El Hussein؛ D.A. Salih* ؛ D. Berkvens؛ D. Geysen
چکیده
  In an attempt to characterize Theileria parva parasites circulating in South Sudan cattle , polymerase chain reaction (PCR)-based assays were carried out using four single copy encoding antigen genes p104, PIM, p150 and p67 in addition to one microsatellite MS321. A total of 20 bovine DNA samples from two locations in South Sudan were included in this study, in addition to two references strains, Muguga and Katete. A total of nine alleles were identified for the polymorphic immunodominant molecule (PIM) locus using restriction fragment length polymorphism (RFLP), against 2 alleles each for the p150 and p104 loci, respectively. This confirms that differences in the polymorphic PIM genes alone could be used to characterize subdivisions in the T. parva populations in the field. On the other hand, 4 alleles were identified for the MS321 locus and 2 alleles for the p67 locus. The data indicate that the studied parasites are genetically closely related, mainly cattle derived and genetically quite distinct from the Muguga strain.
کلیدواژه‌ها
Characterization؛ PCR؛ RFLP؛ South Sudan؛ Theileria parva
عنوان مقاله [English]
شناسایی مولکولی انگل تیلریا پاروا در جنوب سودان با استفاده از آزمون PCR-RFLP بر روی ژن های مولد آنتی ژن
نویسندگان [English]
W.L. Marcellino؛ A.M. El Hussein؛ D.A. Salih؛ D. Berkvens؛ D. Geysen
آمار
تعداد مشاهده مقاله: 2,104
تعداد دریافت فایل اصل مقاله: 3,075


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