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Study on Immunity of an Experimental Oil Adjuvant Haemorrhagic Septicaemia Vaccine in Cattle. | ||
| Archives of Razi Institute | ||
| مقاله 10، دوره 59، شماره 2، فروردین 2005، صفحه 95-101 اصل مقاله (110.18 K) | ||
| شناسه دیجیتال (DOI): 10.22092/ari.2005.103817 | ||
| نویسندگان | ||
| A. Sotoodehnia* ؛ G. Moazeni؛ S. Ataei؛ B. Omidi | ||
| چکیده | ||
| An oil adjuvant vaccine (OAV) was prepared from a local strain of Pasteurella multocida. Strain 6:B was cultured and inactivated by formalin. Bacterial pellet was prepared by centrifugation and subsequently adjuvanted by Montannide oil ISA-70. A dose of prepared vaccine containing 3ml (2mg dry weight/ml) was injected into five calves by IM route. Animals were bled before and at 24, 90, 150, and 200 days post-vaccination. Collected sera were used in passive mouse protection test (PMPT). Active mouse protection test (AMPT) was carried out for OAV according to standard method. Results of PMPT showed 100% protection up to 150 days and 66-83% up to 200 days post-vaccination. In AMPT, 4 log of protection was gained. In this experiment the immunity induced by OAV adjuvanted by ISA-70 could protect the calves. | ||
| کلیدواژه‌ها | ||
| haemorrhagic septicaemia؛ oil adjuvant vaccine؛ Protection | ||
| عنوان مقاله [English] | ||
| مطالعه بر روی ایمنی زایی یک واکسن آزمایشی سپتی سمی هموراژیک با ادجوان روغنی در گاو | ||
| نویسندگان [English] | ||
| ا. ستوده نیا؛ غ. موذنی؛ سعید عطایی؛ ب. امیدی | ||
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