| تعداد نشریات | 284 |
| تعداد نشریات سازمان | 82 |
| تعداد شماره‌ها | 3,044 |
| تعداد مقالات | 34,047 |
| تعداد مشاهده مقاله | 46,440,838 |
| تعداد دریافت فایل اصل مقاله | 77,509,244 |
تاثیر غلظتهای زیرکشنده شیرابه فرفیون ترکمنی (Euphorbia turcomanica) بر برخی شاخص های بیوشیمیایی کبد ماهی آفانیوس گورخری (Aphanius dispar) | ||
| مجله علمی شیلات ایران | ||
| مقاله 3، دوره 23، شماره 4، اسفند 1393، صفحه 31-47 اصل مقاله (1.22 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/isfj.2015.103167 | ||
| نویسندگان | ||
| هما زارع؛ احمد نوری* ؛ مرتضی یوسف‌زادی؛ مهدی بنایی | ||
| چکیده | ||
| در این مطالعه، بررسی تاثیر سمیت زیر کشنده شیرابه گیاه فرفیون ترکمنی(Euphorbia turcomanica) در غلظت های صفر، 0055/0، 011/0 و 022/0 گرم بر لیتر بر روی برخی تغییرات شاخص های بیوشیمیایی کبد ماهی آفانیوس گورخری (Aphanius dispar) پس از گذشت 15 و 30 روز و در شرایط آزمایشگاهی مورد مطالعه قرار گرفت. ابتدا مقدار میانگین (± خطای استاندارد) 50LC شیرابه گیاه فرفیون ترکمنی در زمانهای 24، 48، 72 و 96 ساعت به ترتیب 14/0±28/0، 06/0±19/0، 03/0±14/0، 02/0±11/0 گرم بر لیتر بدست آمد. برخی از شاخص های بیوشیمیایی شامل مقادیر آنزیم آسپارتات آمینوترانسفراز (AST)، آلانین آمینوترانسفراز (ALT)، لاکتات دهیدروژناز (LDH)، کراتین فسفوکیناز (CK)، و آلکالین فسفاتاز (ALP) در بافت کبد ماهیان بعد از گذشت 15 و 30 روز از شروع آزمایش اندازه گیری شد. تغییرات معنیداری در سطوح آنزیم های AST، ALT، LDH، CK، و ALP درمقایسه با گروه کنترل در عصاره بافتی کبد در طول دوره آزمایش، مشاهده شد. این تغییرات به صورت افزایش معنی دار در سطوح آنزیم های AST، LDH و ALP و کاهش معنی دار در میزان فعالیت آنزیم های ALT و CK بود. همچنین روند کاهشی معنی داری در میزان پروتئین کل در عصاره بافت کبد متناسب با زمان در تماس بودن ثبت گردید. با توجه به نتایج این تحقیق، اطلاعات پایه درباره سمیت گیاه فرفیون ترکمنی بر روی ماهی آفانیوس گورخری و همچنین تعیین تاثیرات کاربرد شیرابه حاصل از این گیاه در آب به عنوان راهنما می تواند امکان پذیر باشد. | ||
| کلیدواژه‌ها | ||
| آفانیوس گورخری؛ شیرابه فرفیون ترکمنی؛ غلظت زیرکشنده؛ آنزیم کبدی | ||
| عنوان مقاله [English] | ||
| Effects of sub-lethal concentrations of Euphorbia turcomanica extract on some liver biochemical parameters of Zebra Aphanius (Aphanius dispar) | ||
| نویسندگان [English] | ||
| H. Zare؛ A. Noori؛ M. Yousefzadi؛ M. Banaee | ||
| چکیده [English] | ||
| In the present study, effects of sub-lethal concentrations of Euphorbia turcomanica extract with ranging from 0.00, 0.0055 (5% of LC50), 0.011 (10% of LC50) and 0.022 (20% of LC50) g/lit were investigated over 30 days on biochemical parameters of Zebra Aphanius (Aphanius dispar). The average (±SE) 24, 48, 72 and 96 LC50 rates of E. turcomanica on fish were 0.28±0.14, 0.19±0.06, 0.14±0.03 and 0.11±0.02 g/lit, respectively. The biochemical parameters including aspartate aminotransferase (AST), alanine aminotransferase (ALT), lactate dehydrogenase (LDH), creatine phosphokinase (CK), and alkaline phosphatase (ALP) activities in the liver tissue were measured after 15 and 30 days. Significant changes in AST, ALT, ALP, LDH and CK activities were observed in fish exposed to different concentrations of E. turcomanica extract when compared with control group. The significant increase was determined in AST, LDH and ALP while in ALT and CK, significant decrease was revealed. Also in liver total protein, a significant descending trend related to exposure time was demonstrated. In conclusion, the findings from this study provide basic information about toxicity of E. turcomanica extract on Zabra Aphanius, as well as developing guidelines for evaluating the effects of administration of E. turcomanica derivatives in water. | ||
| کلیدواژه‌ها [English] | ||
| Aphanius dispar, Euphorbia turcomanica, Sub-lethal concentration, Hepatic enzymes | ||
| مراجع | ||
|
بنایی م.، میرواقفی ع.، سوردا گومیل آ.، رفیعی غ. و احمدی ک.، 1391. مطالعه تغییرات فاکتورهای بیوشیمیایی خون و آسیبشناسی بافتی کبد ماهی قزل آلای رنگین کمان ( (Oncorhynchus mykissدر تماس با غلظتهای زیرکشنده دیازینون. نشریه محیط زیست طبیعی، مجله منابع طبیعی ایران، 313-297 ،(3)65. بنایی م.، کیهانی ا. و احمدی، ک.، 1392. تاثیر 2،4-دی کلروفنوکسی استیک اسید (D-4،2) بر برخی پارامترهای بیوشیمیایی و آسیبشناسی بافتی در آبشش و کبد ماهی مولی (Poecilia sphenops). مجله بهرهبرداری و پرورش آبزیان، 105-83 ،(3)2. Adedapo, A.A., Abatan, M.O. and Olorunsogo, O.O., 2004. Toxic effects of some plants in the genus Euphorbia on haematological and biochemical parameters of rats. Veterinarski Arhiv, 74, 53-62. Agrahari, S. and Gopal, K., 2009. Fluctuations of certain biochemical constituents and markers enzymes as a consequence of monocrotophos toxicity in the edible freshwater fish, Channa punctatus. Pesticide Biochemistry and Physiology, 94, 5-9. Akpa, L., Ajima, M., Nwani, C. and Onu, C., 2009. Toxic effect of Euphorbia tirucalli (Pencil tree) sap on Tilapia zillii fingerlings. Environment Conservation Journal, 10, 87-92. Aydın, R. and Köprücü, K., 2005. Acute toxicity of diazinon on the common carp (Cyprinus carpio L.) embryos and larvae. Pesticide Biochemistry and Physiology, 82, 220-225. Banaee, M., 2010. Influence of silymarin in decline of sub-lethal diazinon-induced oxidative stress in rainbow trout (Oncorhynchus mykiss). Ph.D. Thesis, Aquaculture and Environmental Department, Natural Resource Faculty, Natural Resource and Agriculture Collage, Tehran University, Iran. Banaee, M. and Ahmadi, K., 2011. Sub-lethal toxicity impacts of endosulfan on some biochemical parameters of the freshwater crayfish (Astacus leptodactylus). Research Journal of Environmental Sciences, 5, 827-835.
Banaee, M., Mirvagefei, A., Rafei, G. and Majazi Amiri, B., 2008. Effect of sub-lethal diazinon concentrations on blood plasma biochemistry. International Journal of Environmental Research, 2, 189-198.
Banaee, M., Sureda, A., Mirvaghefi, A. and Ahmadi, K., 2011. Effects of diazinon on biochemical parameters of blood in rainbow trout (Oncorhynchus mykiss). Pesticide Biochemistry and Physiology, 99, 1-6.
Banaee, M., Sureda, A., Zohiery, F., Hagi, B.N. and Garanzini, D.S., 2014. Alterations in biochemical parameters of the freshwater fish, Alburnus mossulensis, exposed to sub-lethal concentrations of Fenpropathrin. International Journal of Aquatic Biology, 2, 58-68.
Bhattacharya, H., Xiao, Q. and Lun, L., 2008. Toxicity studies of nonylphenol on rosy barb (Puntius conchonious): A biochemical and histopathological evaluation. Tissue and Cell, 40, 243-249.
Coad, B.W., 1998. Threatened fishes of the world: Lebias ginaonis (Holly, 1929) (Cyprinodontidae). Environmental Biology of Fishes, 51: 284-284.
Coad, B.W. and Abdoli, A., 2000. Systematics of an isolated population of tooth-carp from northern Iran (Actinopterygii: Cyprinodontidae). Zoology in the Middle East, 21, 87-102.
Dagang, W., Sorg, B., Adolf, W., Seip, E. and Hecker, E., 1992. Oligo- and macrocyclic diterpenes in thymelaeaceae and euphorbiaceae occurring and utilized in Yunnan (Southwest China). 2. Ingenane type diterpene esters from Euphorbia nematocypha Hand.-Mazz. Phytotherapy Research, 6, 237-240.
Dickmeis, T., Rastegar, S., Aanstad, P., Clark, M., Fischer, N., Plessy, C., Rosa, F., Korzh, V. and Strähle, U., 2001. Expression of brain subtype creatine kinase in the zebrafish embryo. Mechanisms of development, 109, 409-412.
El-Sayed, Y.S. and Saad, T.T., 2008. Subacute Intoxication of a Deltamethrin-Based Preparation (Butox® 5% EC) in Monosex Nile Tilapia, Oreochromis niloticus L. Basic & Clinical Pharmacology & Toxicology, 102, 293-299.
Fafioye, O., Adebisi, A. and Fagade, S., 2004. Toxicity of Parkia biglobosa and Raphia vinifera extracts on Clarias gariepinus juveniles. African Journal of Biotechnology, 3, 627-630.
Fai, P. and Fagade, S., 2005. Acute toxicity of Euphorbia kamerunica on Oreochromis niloticus. Ecotoxicology and environmental safety, 62: 128-131.
Gabriel, U., Obomanu, F. and Edori, O., 2009. Haematology, plasma enzymes and organ indices of Clarias gariepinus after intramuscular injection with aqueous leaves extracts of Lepidagathis alopecuroides. African Journal of Biochemistry Research, 3, 312-316.
Gong, H.-Y., Wu, J.-L., Huang, W.-T., Ji-Fan Lin, C. and Weng, C.-F., 2004. Response to acute changes in salinity of two different muscle type creatine kinase isoforms, from euryhaline teleost (Oreochromis mossambicus) gills. Biochimica et Biophysica Acta (BBA)-General Subjects, 1675, 184-191.
Grzyb, K. and Skorkowski, E.F., 2005. Characterization of creatine kinase isoforms in herring (Clupea harengus) skeletal muscle. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 140, 629-634.
Gundidza, M., Sorg, B. and Hecker, E., 1993. A skin irritant principle from Euphorbia matabelensis Pax. Journal of Ethnopharmacology, 39, 209-212.
Haagensen, L., Jensen, D.H. and Gesser, H., 2008. Dependence of myosin-ATPase on structure bound creatine kinase in cardiac myfibrils from rainbow trout and freshwater turtle. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 150, 404-409.
Hrbek, T., Keivany, Y. and Coad, B.W., 2006. New species of Aphanius (Teleostei, Cyprinodontidae) from Isfahan Province of Iran and a reanalysis of other Iranian species. Copeia, 2006, 244-255.
Kavitha, C., Ramesh, M., Kumaran, S.S. and Lakshmi, S.A., 2012. Toxicity of Moringa oleifera seed extract on some hematological and biochemical profiles in a freshwater fish, Cyprinus carpio. Experimental and Toxicologic Pathology, 64, 681-687.
Korkmaz, N., Cengiz, E., Unlu, E., Uysal, E. and Yanar, M., 2009. Cypermethrin-induced histopathological and biochemical changes in Nile tilapia (Oreochromis niloticus), and the protective and recuperative effect of ascorbic acid. Environmental Toxicology and Pharmacology, 28, 198-205.
Kumar, A., Prasad, M., Mishra, D., Srivastav, S.K. and Srivastav, A.K., 2010. Toxicity of aqueous extract of Euphorbia tirucalli latex on catfish, Heteropneustes fossilis. Ecotoxicology and environmental safety, 73, 1671-1673.
Kumar, N., Antony Jesu Prabhu, P., Pal, A., Remya, S., Aklakur, M., Rana, R., Gupta, S., Raman, R. and Jadhao, S., 2011. Anti-oxidative and immuno-hematological status of Tilapia (Oreochromis mossambicus) during acute toxicity test of endosulfan. Pesticide Biochemistry and Physiology, 99, 45-52.
Murray, R.K., Granner, D.K., Mayes, P.A. and Rodwell, V., 2003. Harper’s Illustrated Biochemistry, Twenty-Sixth Edition. Lange Medical Books/McGraw-Hill (Medical Publishing Division), New York. 420P.
Mwine, T.J., Van Damme, P., Hastilestari, B.R. and Papenbrock, J., 2013. Euphorbia tirucalli L.(Euphorbiacaea): the miracle tree: current status of knowledge. African natural plant products, volume II: discoveries and challenges in chemistry, health, and nutrition, 1127, 4-17.
Ozawa, E., Hagiwara, Y. and Yoshida, M., 1999. Creatine kinase, cell membrane and Duchenne muscular dystrophy, Muscle Physiology and Biochemistry. Springer US, pp. 143-151.
Patil V.K. and David M., 2008. Behaviour and respiratory dysfunction as an index of malathion toxicity in the freshwater fish, Labeo rohita (Hamilton). Turkish Journal of Fisheries and Aquatic Sciences, 8, 233-237.
Petrovic, S., Ozretiç, B. and Krajnovic-Ozretic, M., 1996. Cytosolic aspartate aminotransferase from the grey mullet (Mugil auratus Risso) red muscle: Isolation and properties. The International Journal of Biochemistry & Cell Biology, 28, 873-881.
Rajeh, M.A.B., Kwan, Y.P., Zakaria, Z., Latha, L.Y., Jothy, S.L. and Sasidharan, S., 2012. Acute toxicity impacts of Euphorbia hirta L extract on behavior, organs body weight index and histopathology of organs of the mice and Artemia salina. Pharmacognosy research, 4,170.
Rao, J.V., 2006. Toxic effects of novel organophosphorus insecticide (RPR-V) on certain biochemical parameters of euryhaline fish, Oreochromis mossambicus. Pesticide Biochemistry and Physiology, 86: 78-84.
Reichenbacher, B., Kamrani, E., Esmaeili, H.R. and Teimori, A., 2009. The endangered cyprinodont Aphanius ginaonis (Holly, 1929) from southern Iran is a valid species: evidence from otolith morphology. Environmental Biology of Fishes, 86, 507-521.
Saha, S. and Kaviraj, A., 2009. Effects of cypermethrin on some biochemical parameters and its amelioration through dietary supplementation of ascorbic acid in freshwater catfish Heteropneustes fossilis. Chemosphere, 74, 1254-1259.
Saravanan, M., Prabhu Kumar, K. and Ramesh, M., 2011. Haematological and biochemical responses of freshwater teleost fish Cyprinus carpio (Actinopterygii: Cypriniformes) during acute and chronic sublethal exposure to lindane. Pesticide Biochemistry and Physiology, 100, 206-211.
Shanmugam, S., Sathish Kumar, T. and Panneer Selvqam, K., 2010. Laboratory Handbook on Biochemistry. Asoke K. Ghosh, PHI Learning Private Limited, M-97New Delhi-110001. 141 P.
Singh, S.K., Yadav, R.P., Singh, D. and Singh, A., 2004. Toxic effect of two common Euphorbiales latices on the freshwater snail Lymnaea acuminata. Environmental Toxicology and Pharmacology, 15, 87-93.
Srivastava, A.S., Oohara, I., Suzuki, T., Shenouda, S., Singh, S.N., Chauhan, D.P. and Carrier, E., 2004. Purification and properties of cytosolic alanine aminotransferase from the liver of two freshwater fish, Clarias batrachus and Labeo rohita. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 137, 197-207.
Tiwari, S. and Singh, A., 2003. Control of common freshwater predatory fish, Channa punctatus, through Nerium indicum leaf extracts. Chemosphere, 53, 865-875.
Tiwari, S. and Singh, A., 2004. Piscicidal and anti-acetylcholinesterase activity of Euphorbia royleana stem bark extracts against freshwater common predatory fish Channa punctatus. Environmental Toxicology and Pharmacology, 18, 47-53.
Tiwari, S. and Singh, A., 2006. Biochemical stress response in freshwater fish Channa punctatus induced by aqueous extracts of Euphorbia tirucalli plant. Chemosphere, 64, 36-42.
Tiwari, S. and Singh, A., 2009. Changes in some biochemical parameters in the liver and muscle of Colisa fasciatus due to toxicity of ethanolic extract of Nerium indicum Mill.(Lal Kaner) latex. Natural Product Radiance, 8, 48-54.
Velišek, J., Svobodova, Z. and Machova, J., 2009. Effects of bifenthrin on some haematological, biochemical and histopathological parameters of common carp (Cyprinus carpio L.). Fish Physiology and Biochemistry, 35, 583-590.
Velíšek, J., Dobšíková, R., Svobodova, Z., Modra, H. and Luskova, V., 2006. Effect of deltamethrin on the biochemical profile of common carp (Cyprinus carpio L.). Bulletin of Environmental Contamination and Toxicology, 76: 992-998.
Vutukuru, S.S., Prabhath, N.A., Raghavender, M. and Yerramilli, A., 2007. Effect of arsenic and chromium on the serum amino-transferases activity in Indian major carp, Labeo rohita. International journal of environmental research and public health, 4, 224-227.
Wells, R., McIntyre, R., Morgan, A. and Davie, P., 1986. Physiological stress responses in big gamefish after capture: observations on plasma chemistry and blood factors. Comparative Biochemistry and Physiology Part A: Physiology, 84, 565-571.
| ||
|
آمار تعداد مشاهده مقاله: 1,026 تعداد دریافت فایل اصل مقاله: 1,417 |
||