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بررسی مقایسهای نگهداری برخی جلبکهای آب شیرین به روشهای انجماد خشک و محافظت سرمایی | ||
| مجله علمی شیلات ایران | ||
| مقاله 10، دوره 24، شماره 2، آذر 1394، صفحه 103-114 اصل مقاله (592.2 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/isfj.2015.103143 | ||
| نویسندگان | ||
| الهام فیاضی آتدوتان؛ هومن رجبی اسلامی* | ||
| چکیده | ||
| این تحقیق به منظور بررسی امکان نگهداری جلبکهای آب شیرین به روش انجماد خشک و محافظت سرمایی و مقایسه قابلیت رشد آنها انجام شد. 3 گونه جلبک آزمایشی شامل Chlamydomonas moewusii، Scenedesmus obliquusو Chlorococcum olefaciens پس از تهیه محیط کشت زایندر در 6 ارلن به مدت 20 روز کشت داده شدند. سپس هر گونه با نه تکرار (سه تکرار برای هر یک از روزهای اول، پنجم و دهم) به روشهای انجماد خشک و محافظت سرمایی برای نگهداری آماده گردید. جلبکها در ادامه، کشت مجدد شده و رشد آنها با سنجش کلروفیل a طی روزهای اول، پنجم و دهم پس از خروج از شرایط حفاظتی بررسی گردید. تعداد 9 ارلن نیز برای هر گونه جلبکی تنها با افزودن محیط کشت اختصاصی جلبک به عنوان تیمار شاهد آماده گردید تا مقیاس مشابهی برای بررسی رشد جلبکها در روزهای یکسان فراهم آید. نتایج نشان داد که بیشترین رشد هر سه گونه در انجماد خشک پس از گذشت ده روز از کشت مجدد به دست آمد که به شکل معنیداری (p<0.05) بالاتر از تیمار شاهد بود. با این وجود تفاوت معنیداری در میزان رشد ScenedesmusobliquusوChlorococcumolefaciensپس از محافظت سرمایی و انجماد خشک به دست نیامد (p>0.05). یافتههای پژوهش حاضر نشان داد که هر دو روش حفاظتی برای نگهداری سه گونه جلبک سبز مطالعاتی مناسب است، گرچه روش انجماد خشک در گونه Chlamydomonasmoewusii نتایج بهتری را ارایه نمود. | ||
| کلیدواژه‌ها | ||
| Chlamydomonas moewusii؛ obliquus Scenedesmus؛ Chlorococcum olefaciens؛ انجماد خشک؛ محافظت سرمایی | ||
| عنوان مقاله [English] | ||
| Comparison of some freshwater green algae conservation using the of freeze-drying and cryopreservation methods | ||
| نویسندگان [English] | ||
| E Fayazi Atdotan؛ H Rajabi Islami | ||
| چکیده [English] | ||
| This study was conducted to evaluate the possible conservation of freshwater green algae using the freeze-drying and cryopreservation methods and comparative of their growth potential. Three experimental algae including Chlamydomonas moewusii, Scenedesmus obliquus, and Chlorococcum olefaciens were cultured for 20 days in 6 Erlenmeyer flasks after preparation of Zinder growth medium. Each algal strain was preserved according to freeze-drying and cryopreservation protocols with 9 replications (3 replications for the days of 1, 5, and 10). Algae were then re-incubated and their growth was evaluated in 1, 5, and 10 days after preservation. Another 9 Erlenmeyer flasks for each algal species were prepared as control treatment by adding the respective growth medium to consider the growth of algae in the same days with similar scale. Highest growth of all experimental algae was obtained after 10 days which was significantly higher than the corresponding control treatments (p<0.05). However, no significant difference was observed in the growth of Scenedesmus obliquus andand Chlorococcum olefaciens after freeze-drying and cryopreservation (p>0.05). Results of the present study illustrated that both preservative methods are suitable for maintenance of experimental algae, although freeze-drying provided better results in Chlamydomonasmoewusii. | ||
| کلیدواژه‌ها [English] | ||
| Chlamydomonas moewusii, Freeze- drying, SCENEDESMUS OBLIQUUS, Chlorococcum olefaciens, Freeze- drying, Cryopreservation | ||
| مراجع | ||
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