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GENETIC VARIATION ANALYSIS OF DIFFERENT POPULATIONS OF ROSA DAMASCENA IN NW. IRAN USING RAPD MARKERS | ||
| The Iranian Journal of Botany | ||
| مقاله 3، دوره 12، شماره 2، اسفند 2006، صفحه 121-127 اصل مقاله (302.39 K) | ||
| نوع مقاله: Research Paper | ||
| نویسندگان | ||
| Seyed Reza Tabaei-Aghdaei1؛ Hossein Hosseini Monfared2؛ Hamid Fahimi3؛ Hassan Ebrahimzade3؛ Maryam Jebelly1؛ Mohammad Reza Naghavi4؛ Alireza Babaei5 | ||
| 1Biotechnology Research Department of Natural Resources, Research Institute of Forests and Rangelands, P.O. Box 13185-116, Tehran, Iran. | ||
| 2Zanjan Province Natural Resources Office, Zanjan, Iran. | ||
| 3Depaetment of Biology, Tehran University, Tehran, Iran. | ||
| 4Department of Plant Breeding, Tehran University, Tehran, Iran. | ||
| 5Department of Horticultural Science, Tabiat Modares University(TMU), Tehran, Iran. | ||
| چکیده | ||
| Random amplified polymorphic DNA (RAPD) markers have been used to characterize the genetic diversity among 12 Damask rose landraces from northwest of Iran. The 22 primers used in this study amplified 262 scorable RAPD loci among which 180 were polymorphic (67%). The average number of bands was 11.9 for each primer. Genetic similarity calculated from the RAPD data ranged from 0.261 to 0.995 with an average of 0.63. A dendrogram was prepared on the basis of a similarity matrix using the UPGMA algorithm and separated the 12 landraces into two groups. There was a good correspondence between genetic divergence and climate condition, though some landraces (such as L2 and L3) with diverse geographical condition were clustered in one group. The results showed a possible application of RAPD markers in assessment of genetic diversity in Rosa damascena landraces. | ||
| کلیدواژه‌ها | ||
| Rosa damascena Mill؛ genetic similarity؛ RAPD analysis | ||
| عنوان مقاله [English] | ||
| بررسی تنوع ژنتیکی جمعیت های مختلف گل محمدی شمال غرب ایران با استفاده از نشانگر RAPD | ||
| نویسندگان [English] | ||
| سید رضا طبائی عقدائی1؛ حسین حسینی منفرد2؛ حمید فهیمی3؛ حسن ابراهیم زاده3؛ مریم جبلی1؛ محمدرضا نقوی4؛ علیرضا بابائی5 | ||
| 1گروه تحقیقات بیوتکنولوژی موسسه تحقیقات جنگلها و مراتع، تهران، ایران | ||
| 2منابع طبیعی زنجان، زنجان، ایران | ||
| 3دانشگاه تهران، تهران، ایران | ||
| 4گروه اصلاح نباتات، دانشگاه تهران، تهران، ایران. | ||
| 5گروه علوم باغبانی، دانشگاه تربیت مدرس، تهران، ایران. | ||
| چکیده [English] | ||
| در این پژوهش نشانگر مولکولیRAPD برای تعیین تنوع ژنتیکی 12 جمعیت گل محمدی شمال غرب ایران مورد استفاده قرار گرفت. با استفاده از 22 آغازگر، تعداد 262 باند قابل تشخیص ایجاد شد که 67% آنها ( 180 باند) در بین جمعیتهای مختلف چند شکلی نشان دادند. تعداد متوسط باندها به ازای هر آغازگر 9/11 بود. شاخص تشابه Dice برای تعیین همسانیهای ژنتیکی بین جمعیتها مورد استفاده قرار گرفته و با استفاده از الگوریتم UPGMA یک دندروگرام بر مبنای ماتریس تشابه تهیه شد که 12 جمعیت گل محمدی را در2 گروه مختلف قرار داد. نتایج بدست آمده ارتباط خوبی بین تنوع آب و هوایی و مولکولی نشان دادند، گرچه جمعیتهای با تنوع جغرافیایی (مانند L2 وL3) نیز در یک گروه قرار گرفتند. نتایج این پژوهش بر امکان بکارگیری نشانگرهای RAPD در ارزیابی تنوع ژنتیکی ژرم پلاسم گل محمدی تاکید داشت. | ||
| کلیدواژه‌ها [English] | ||
| Rosa damascena Mill, genetic similarity, RAPD analysis | ||
| مراجع | ||
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