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Bakhshi, Behnam, Aghaei, Mohammad Jaffar, Bihamta, Mohamad Reza, Darvish, Farrokh, Zarifi, Eissa. (1389). PLOIDY DETERMINATION OF AEGILOPS CYLINDRICA HOST ACCESSIONS OF IRAN BY USING FLOW CYTOMETRY AND CHROMOSOME COUNTING. سامانه مدیریت نشریات علمی, 16(2), 258-266.
Behnam Bakhshi; Mohammad Jaffar Aghaei; Mohamad Reza Bihamta; Farrokh Darvish; Eissa Zarifi. "PLOIDY DETERMINATION OF AEGILOPS CYLINDRICA HOST ACCESSIONS OF IRAN BY USING FLOW CYTOMETRY AND CHROMOSOME COUNTING". سامانه مدیریت نشریات علمی, 16, 2, 1389, 258-266.
Bakhshi, Behnam, Aghaei, Mohammad Jaffar, Bihamta, Mohamad Reza, Darvish, Farrokh, Zarifi, Eissa. (1389). 'PLOIDY DETERMINATION OF AEGILOPS CYLINDRICA HOST ACCESSIONS OF IRAN BY USING FLOW CYTOMETRY AND CHROMOSOME COUNTING', سامانه مدیریت نشریات علمی, 16(2), pp. 258-266.
Bakhshi, Behnam, Aghaei, Mohammad Jaffar, Bihamta, Mohamad Reza, Darvish, Farrokh, Zarifi, Eissa. PLOIDY DETERMINATION OF AEGILOPS CYLINDRICA HOST ACCESSIONS OF IRAN BY USING FLOW CYTOMETRY AND CHROMOSOME COUNTING. سامانه مدیریت نشریات علمی, 1389; 16(2): 258-266.

PLOIDY DETERMINATION OF AEGILOPS CYLINDRICA HOST ACCESSIONS OF IRAN BY USING FLOW CYTOMETRY AND CHROMOSOME COUNTING

The Iranian Journal of Botany
مقاله 16، دوره 16، شماره 2، اسفند 2010، صفحه 258-266    اصل مقاله (1.15 M)
نوع مقاله: Research Paper
نویسندگان
Behnam Bakhshi* 1؛ Mohammad Jaffar Aghaei2؛ Mohamad Reza Bihamta3؛ Farrokh Darvish4؛ Eissa Zarifi2
1Young Research Club, Science and Research Branch, Islamic Azad University, Tehran, Iran
2Seed and Plant Improvement Institute, National Plant Gene Bank of Iran, Karaj, Iran
3College of Agriculture and Natural Resources, Tehran University
4Islamic Azad University, Science and Research Branch, Tehran, Iran
چکیده
Chromosomes counting and flow cytometry method were used for assessing the ploidy level of various Aegilops cylindrica Host (2n=4x=28, CCDD) accessions of the National Plant Gene Bank of Iran (NPGBI). About 100 different accessions of Ae. cylindrica were estimated in flow cytometry analysis by using Ae. tauschii as  a control sample. All the studied accessions fitted in 4x ploidy level. Chromosomes counts and flow cytometry data led to the same results. There was significant diversity in DNA index among Ae. cylindrica accessions. Cluster analysis was used for the classification of Ae. cylindrica based on their DNA content. Interestingly, accessions with smaller and larger DNA index had smaller and larger chromosome length, respectively. Also, the study of the relationship of DNA content and geographical distribution in Iran showed that there is diversity in DNA content of  Ae. cylindrica  for different areas of Iran.
کلیدواژه‌ها
Aegilops cylindrica؛ Flow cytometry؛ Ploidy level؛ chromosomes counting؛ Iran
عنوان مقاله [English]
تعیین سطح پلوئیدی گونه Aegilops cylindrica در ایران از طریق فلوسایتومتری و شمارش کروموزومی
نویسندگان [English]
بهنام بخشی1؛ محمد جعفر آقایی2؛ محمد رضا بی همتا3؛ فرخ درویش4؛ عیسی ظریفی2
1محقق، باشگاه پژوهشگران جوان، دانشگاه آزاد اسلامی، واحد علوم و تحقیقات
2استادیار، موسسه تحقیقات اصلاح و تهیه نهال بذر
3استاد، پردیس کشاورزی و منابع طبیعی دانشگاه تهران
4استاد، دانشگاه آزاد اسلامی، واحد علوم و تحقیقات
چکیده [English]
در این تحقیق از شمارش کروموزومی و روش فلوسیتومتری برای تخمین سطح پلوئیدی نمونه های مختلفAegilops cylindrica Host (2n = 4x = 28, CCDD) موجود در بانک ژن گیاهی ملی ایران استفاده شد. تعداد 100 نمونه  Ae. cylindrica در آنالیز فلوسیتومتری با استفاده از Ae. tauschiiبه عنوان نمونه کنترل مورد استفاده قرار گرفتند. تمام نمونه های مورد بررسی دارای سطح پلوئیدی 4x بودند. نتایج حاصل از فلوسیتومتری و شمارش کروموزومی مشابه بود. تنوع معنی داری در شاخص DNA نمونه های Ae. cylindrica وجود داشت. تجزیه خوشه ای برای طبقه بندی Ae. cylindricaبر اساس محتوای DNA آنها انجام شد. به طور جالبی، نمونه های با شاخص DNA کوچک و بزرگ به ترتیب دارای طول کروموزوم کوتاه و طویل بودند. همچنین، مطالعه رابطه بین محتوای DNA و پراکنش جغرافیایی در ایران نشان داد که برای Ae. cylindrica در مناطق مختلف ایران از لحاظ محتوای DNA تنوع وجود دارد.
کلیدواژه‌ها [English]
Aegilops cylindrica, Flow cytometry, Ploidy level, chromosomes counting, Iran
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