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جداسازی و شناسایی قارچ‌های اندومیکوریزی موجود در ریزوسفر درختان بادام (Amygdalus scoparia Spach) در جنگلهای زاگرس (مطالعه موردی: ذخیرهگاه جنگلی کلم در استان ایلام) | ||
| تحقیقات حمایت و حفاظت جنگلها و مراتع ایران | ||
| مقاله 5، دوره 13، شماره 1، خرداد 1394، صفحه 46-56 اصل مقاله (462.74 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijfrpr.2015.102392 | ||
| نویسندگان | ||
| جواد میرزایی* 1؛ نجمه نوربخش2 | ||
| 1استادیار، گروه علوم جنگل، دانشکده کشاورزی، دانشگاه ایلام | ||
| 2دانشجوی کارشناسی ارشد جنگلداری، دانشگاه ایلام | ||
| چکیده | ||
| این تحقیق بهمنظور شناسایی قارچ‌های میکوریزی با درختان بادام (Amygdalus scoparia) در ذخیرهگاه جنگلی کلم در استان ایلام، انجام شد. بدین منظور در ریزوسفر درختان بادام نمونه‌های خاک از عمق 0-30 سانتی‌متری تهیه شد. آنگاه نمونه‌های خاک پس از انتقال به آزمایشگاه در هوای آزاد خشک شده و بعد اسپور قارچها به روش الک تر و سانتریفیوژ جدا شد. پس از استخراج اسپور قارچها، اسلایدهای دائمی تهیه گردید و قارچها با استفاده از ویژگیهای مرفولوژیکی شناسایی شدند. در این مطالعه، 13 گونه قارچ میکوریز آربوسکولار متعلق به هفت جنس: Funneliformis، Acaulospora،Gigaspora ،Septoglomus ،Diversispora ، Claroideoglomusو Rhizophagus در ریزوسفر بادام شناسایی شدند که جنس Rhizophagus با 8/30 درصد، جنس غالب در منطقه بود. علاوهبراین گونه‌های Funneliformis caledonius، F. mosseae، Rhizophagus intraradices، R. fasciculatus ،R. clarum، Claroideoglomus drummondii و Gigaspora gigantea بیشترین فراوانی و گونه‌ی C. etunicatumکمترین فراوانی را در ریزوسفر بادام داشتند. همچنین بیشترین فراوانی اسپور قارچ، متعلق به گونه Funneliformis mosseaeبود. | ||
| کلیدواژه‌ها | ||
| بادام کوهی؛ قارچ؛ اندومیکوریز؛ اکوسیستم؛ ایلام | ||
| عنوان مقاله [English] | ||
| Isolation and identification of arbuscular mycorrhiza fungi in the roots and rhizosphere of Amygdalus scoparia Spach in Zagros forests (Case study: Kolm reservoir in Ilam province) | ||
| نویسندگان [English] | ||
| Javad Mirzaei1؛ Najme Noorbakhsh2 | ||
| 1Assistant Professor, Department of Forest Science, Faculty of Agriculture, Ilam University, Ilam, Iran | ||
| 2M.sc student, forestry, Department of Forest Science, Faculty of Agriculture, Ilam University, Ilam, Iran. | ||
| چکیده [English] | ||
| This study was conducted to identify the arbuscular mycorrhizal fungi in the roots and rhizosphere of Amygdalus scoparia in the forests of Kolm reservoir in Ilam province. Therefore, in the rhizosphere of almond trees, soil and root samples were collected from a depth of 0-30 cm. Soil samples for isolation and identification of fungi were transported to the laboratory and spores of arbuscular fungi were isolated using the wet sieving and decanting method on Sucrose followed by centrifuging. Isolated spores were mounted on slides and Spore morphological characteristics were considered for identification. In total, 13 species belonging to 7 genera of arbuscular mycorrhizal fungi including Funneliformis, Acaulospora, Gigaspora, Septoglomus, Diversispora, Claroideoglomus, Rhizophagus, were identified in the rhizosphere of almond trees. Rhizophagus was the dominant genus at 30.8 percent. Further, the Funneliformis caledonius, F. mosseae, Rhizophagus intraradices, R. fasciculatus, R. clarum, Claroideoglomus drummondii, Gigaspora gigantea were the most and C. etunicatum was the least found species in the area. Most abundance of spores belonged to the Funneliformis mosseae species. | ||
| کلیدواژه‌ها [English] | ||
| Amygdalus scoparia, fungi, endomycorrhizal fungi, Ecosystem, Ilam | ||
| مراجع | ||
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