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همهگیرشناسی بیماری پژمردگی ورتیسیلیومی پنیه: تنوع بیماریزایی جدایههای قارچ Verticillium dahliae | ||
| پژوهش های پنبه ایران | ||
| مقاله 5، دوره 2، شماره 2، آبان 1393، صفحه 59-73 اصل مقاله (389.27 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/ijcr.2015.102033 | ||
| نویسنده | ||
| مرتضی عرب سلمانی* | ||
| استادیار پژوهش مرکز تحقیقات کشاورزی و منابع طبیعی تهران | ||
| چکیده | ||
| به منظور تعیین تنوع عامل بیماری پژمردگی ورتیسیلیومی پنبه، تعداد 95 جدایه قارچ Verticillium dahliae Kleb. از خاک، پنبه، بــادنجـان، بامیه، خیار، پسته، کنجد، گوجه فرنگی و غوزک و ریزوسفر علفهای هرز داتوره، توق و تاجریزی سیاه جمعآوری شدند. خصوصیات بیماریزایی، مورفولوژیکی، بهترین دمای رشد و دامنه میزبانی وسازگاری رویشی جدایهها بررسی گردید. نتایج نشان دادکه جدایهها از نظر ایجاد علائم در پنبه و بامیه به دو گروه برگریز و غیر برگریز تقسیم شدند. جدایههایی که باعث برگریزی در پنبه شدند از نظر علائم در پنبه و بامــیه، مورفولوژی میکرواسکلروت در آب آگار، دامنه میزبانی، دمای بهینه رشد در محیط کشت PDA و سازگاری رویشی با جدایههای غیر برگریز متفاوت بودند. تنوع بیماریزایی درجدایههای غیر برگریز بیشترتر از جدایههای برگریز بود. بیماریزایی متقابل جدایههای جدا شده از میزبانهای مختلف نشان داد که اختصاصیت میزبانی در بین جدایههای مورد مطالعه وجود نداشت ولی شدت بیماریزایی متفاوت بود. سویه برگریز V. dahliae از پنبه، بادنجان و خاک از مناطق پنبهکاری خیر استهبان، کردکوی و ورامین جداسازی و مورد شناسایی قرار گرفت. پنجاه و دو موتانت از 95 جدایه قارچ بدست آمد. بر اساس توان استفاده از منبع نیتروژن موتانتها به سه گروه نیت 1 (25%)، نیت 3 (12 %) و نیت ام (54 %) تقسیم شدند. نتایج نشان دادکه بر اساس سیستم میزبان ـ پاتوژن و علائم ایجاد شده در پنبه دو سویه برگریز و غیر برگریز ولی بر اساس سازگاری رویشی هشت گروه دربین جدایههای مورد مطالعه وجـــود دارد. ســویه برگریز در یک گروه و سویه غیر برگریز در هفت گروه دیگر سازگاری رویشی قرار گرفتند. | ||
| کلیدواژه‌ها | ||
| پژمردگی ورتیسیلیومی؛ سازگاری رویشی؛ تنوع بیماریزایی و پنبه | ||
| عنوان مقاله [English] | ||
| Epidemiology of verticillium wilt of cotton: Pathogenicity variation among isolates Verticillium dahlia | ||
| نویسندگان [English] | ||
| M. Arabsalmani | ||
| Assistant Prof. Agricultural and Natural Resources Research Center of Tehran Province | ||
| چکیده [English] | ||
| In order to determination of variability of pathogenicity of Verticillium dahliae kleb., Ninety five isolates form soil, cotton, eggplant, okra, cucumber, pistachio, sesame, tomato, and wild hemp, and rhizospher of Datura stramonium, Xanthium sp. and Solanum nigrum were compared in respect to microsclerotial morphology,pathogenicity, host range, optimum temperature for growth and vegetative compatibility groups (VCGs).The result showed that based on symptom in cotton and okra plants the isolates were divided in two defoliating and no defoliating strains which also differed in microsclerotial morphology on water agar, optimal temperature for growth on potato dexteros agar. The variability among no defoliating isolates were more than defoliating isolates. Although, there was not host specificity among isolates but observed various disease severity in cotton .The defoliating strains were collected from cotton, eggplant, sesame and soil in Estahban, Kord kuy and Varamin regions. From 52 nit mutants (54%) were obtained 95 isolates. Based on the ability to use different nitrogen sources the mutant were grouped into three phenotypic classes nit 1(25%), nit 3(12%) and nit M( 54%).The isolates were separated into different groups. Eight VCGs were recognized among nit mutants. All defoliating isolates were belonged to one VCG and rest of the isolated grouped in 7 VCGS. | ||
| کلیدواژه‌ها [English] | ||
| Verticillium wilt, cotton, Vegetative Compatibility Groups | ||
| مراجع | ||
|
Ahoomanesh, A. 2007. Principles of Plant Disease Control. Isfahan University of
Technology, 391pp.
Arabsalman I., Okhovvat, S.M., Sharifitherani, A., Nikkha, M.J., and Safaie, N.
2011. Epidemiology of verticillium wilt of cotton in Golestan provience: Effect
of verticillium wilt on quantitative and qualitative characters of cotton. Iranian
J. Plant Pathol. 7(1):1-18.
Arabsalmani, M., and Banihashemi, Z. 2000. Vegetative compatibility groups
within isolates of Verticillium dahliae from cotton and other sources in Iran.
14th Iranian Plant Protection Congress.
Ausher, R., Katan, J., and Ovadia, S. 1975. An improved selective medium for the
isolation of Verticillium dahliae. Phytoparasitica 3: 193-137.
Bhat, R.G., aND Subbarao, V. 2003. Characterization of Verticillium dahliae
isolates and epidemics of pepper. Plant Disease, 87:789-797.
Blanco-Lopea, M.A, Melero-Vara, J.M., and Jimmenz– Diaz, R.M. 1988. Studies
on Verticillium dahliae wilt of cotton in Andalucía Southern Spain.
Agronimique Mediteranian de Montpellier. 3-4 Mars.
Begarano-Alcazar, J., Melero-Vara, J.M., Blanco-Lopez, M.A., and Jimenez-Diaz,
R.M. 1995. Influence of inoculums density of defoliating and non defoliating
pathotypes of Verticillium dahliae on epidemiology of Verticillium of cotton in
Southern Spain .Phytopathology 85: 1474-1481.
Correll, J.C., Gordon, T.R. and Mclain, A.H. 1988. Vegetative compatibility and
pathogenicity of Varticillium albo- atrum. phytophathology, 78:1017-1021.
Cammpbell, C.L., and Madden, L.V. 1990. Introduction to Plant Disease
Epidemiology. New York, WILEY Press, 531p.
Chen, B., Li, S.K., Wang, K.R., Wang, J., Wang, F.Y., Xiae, C.H., Lai, J.C., and
Wang, N. 2008. Spectrum characteristics of cotton canopy infected with
Verticillium wilt and applications. Agri. Sci. in China, 7(5):561–569.
Chang, R.J., and Eastburn, D.M. 1994. Host range of Verticillium dahliae from
hoseradish and pathogeniecty of strains. Plant Dis. 78: 503 - 506.
Carder, J.H., and Barbara, D.J. 1994. Molecular variation within some Japanese
isolates of Verticillium dahliae. Plant Phathol. 43: 947-950.
El-zik, K.M. 1985. Integrated control of Verticillium wilt of cotton. plant Dis.
69:1025 -1032.
Elena, K. 1999. Genetic relationship among Verticillium dahliae from cotton in
Greace based on Vegetative compatibility .Europe. J. Plant Pathol. 105: 609-616.
Found, D., Michel, N., and Jeam, P.G. 1995. Differentiation of Verticillium dahliae
population on the basis of Vegetative compatibility and pathogenicity on cotton.
Europe. J. Platholo. 101: 69-79.
Joaquim, T.F., and Row, R.C. 1990. Reassessment of vegetative compatibility
relationship among strains of Verticillum dahliae using nitrate non-utilizing
mutants. Phytopathol. 80: 1160 -1166.
Hamidi, H., Naderi-Aarefi, A., Forghani, S.H., Arabsalmani, M., Vafai-Tabar, M.,
and Hakimi, M. 2012. Cotton seed production and technology. SPCRI press,
648p.
Howel, C.R. 1970. Differential enzyme synthesis by haploid and diploid forms of
Verticillium albo– atrum. Phytopthol. 60: 488- 490.
Hastie, A.C. 1967. Mitotic recombination of Verticillium albo- atrum. Nature,
214:24- 252.
Hillocks, R.J. 1992. Cotton Diseases. CAB International, Wallingford, UK. 415p.
Horiuchi, S.H., and Takeuchi, S. 1990. Host specificity of isolates of Varticillium
dahliae towards Cruciferous and Solanaceous plants. pp. 235-295. In: Hornby, D.
(ed.), Biological control of Soil born plant pathogens. CAB International UK.
Heale, J. and Isaac, I. 1963. Wilt of Lucerne caused dy species of verticillium IV.
Pathogenicity of Verticillium albo-atrum to Lucerne and other crops: spread and
survival of Verticillium albo- atrum in soil and in weed. Effect upon Lucerne
production. Ann. Appl. Biol. 52: 439- 451.
Isaac, I. 1967. Speciation in verticillium. Annu. Rev. Phytopathol. 5:201–222.
Kirkpatrik, T.L., and Rotrock, C.S. 2001. Compendium of cotton disease. APS
Press, 77 p.
Mace, M.E., Bell, A.A., ad Beckman, C.H. 1981. Fungal wilt disease of Plant.
Academic Press. New York, 640 pp.
Mathere, D.E., Erwin, D.C., Paulus, A.O., and Ravencroft, A.V. 1966. Comparison
of the virulence of isolates of Verticillium albo - atrum from several of the
cotton growing regions in the United States. Plant Dis. Reporter, 50: (12) 930 -
933.
Nelson, R. 1950. Verticillium wilt of Peppermint. Mich. Agr. Eept. Sta. Tech. Bull.
221.
Otero, L., Ducasse, D., and Miller, G. 2004. Variability in ribosomal DNA genetic
and spacer regions in Verticillium dahliae isolates from different hosts.
Fitopatologia Brasileria, 29: 441–446.
Okoli, C.A.N., Carder, J.H. and Barbara, D.J. 1994. Restriction fragment Ineght
polymorphism (RFLPS) and the relationships of some host - adapted isolates of
Verticillium dahliae. Plant pathol. 43: 33-40.
Puhalla, J.E. 1979. Classification of isolates of Verticillium dahliae based on
heterocaryon incompatibility. Phytopathol. 69: 1186–1189.
Puhalla, J.E. and Hummel, M. 1983. Vegetative compatibility groups within
Verticillium dahliae. Phytopathol. 73: 1305 - 1308.
Presely, J.T. 1969. Growth response of Verticillium albo-atrum to sanguinarine in
nutrient agar. Phytopatholo. 59: 1968-1969.
Radisk, S., Jakes, J., Simoni, A., and Javornik, B. 2003.Charactrization of
verticillium albo-atrum field isolates using pathogenicity data and AFLP
analysis. Plant Dis. 87: 633- 638.
Roozbeh, M., and Banihashemi, Z. 2006. Host range of Verticillium dahliae from
Iran. 17th Iranian Protection Congress, 2-5 Sep. p. 428.
Roozbeh, M., and Banihashemi, Z. 2006. Genetic diversity and host specificity of
Verticillium dahliae in Iran. Iranian J. Plant pathol. 42(1):323-325.
Saneii, S.J., RAazavi, S.E., Okhovvat, S.M., and Pahlevani, M. 2010. Verticillium
Wilts. Paek Raihan press. 651pp.
Srinivasan, K.V. 1994. Cotton diseases. Indian Society for Cotton Improvement
C/O CIRCOT.187 p.
Schnathorst, W.C., and Mather, D.E. 1966. Host range and differentiation of a
severe form of Verticillium albo-atrum in cotton. Phytopathol. 56: 1155 – 1165.
Singlton, L.L., Miral, J.D., and Rush, C.M. 1992. Methods for Research on Soilborn
phytopathologenic Fungi. Am. Phytopathol. Soc. St. Minnesota, 265p.
Tjamos, E.C., Rowe, R.C., Heale, J.B., and Fravel, D.R. 2000. Advances in
verticillium research and disease management. Am. Phytopathol. Soc. St.
Minnesota, 357pp.
Watkinson, G.M .1981. Compendium of cotton disease. APS Press, 87 p .
Zare, R. 2001. An integrated approach to the taxonomy of plant-associated
Verticillium species. Rostaniha, 2: 65-66.
Zare, R., and Gams, W. 2000. A revision of Verticillium sect. Prostrata1.
Phylogenetic using its sequences. Nova Hedwigia, 71: 465-480.
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