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افزایش تولید آنتیبیوتیک تایلوزین در باکتری Streptomycess fradiae با استفاده از روش موتاسیون | ||
| تحقیقات دامپزشکی و فرآوردههای بیولوژیک | ||
| شناسنامه علمی شماره، دوره 22، شماره 3، مهر 1388، صفحه 40-47 اصل مقاله (512.04 K) | ||
| نویسندگان | ||
| داود زارع* ؛ مهرداد آذین؛ حسین راثی؛ سیدسعید میردامادی | ||
| عضو هیئت علمی پژوهشکده بیوتکنولوژی | ||
| چکیده | ||
| در این بررسی از دو موتاژن اشعه uv با طول موج nm 280 و (EMS (Ethylmethanesulfonate با غلظتهای متفاوت برای ایجاد موتاسیون و افزایش تولید تایلوزین در باکتری Streptomycess fradiae استفاده شد. منحنی مرگ برای هر دو موتاژن رسم گردید و شرایط بهینه برای ایجاد موتاسیون توسط اشعه uvبا فاصله 20 سانتیمتری و مدت زمان 40 تا 60 ثانیه و برای EMS مدت زمان 30 دقیقه و غلظت (w/v) 4 درصد تشخیص داده شد. در مرحله اول، موتاسیون با uv انجام شد و سویههای دارای تولید بالا به روش پلاک آگار ارزیابی شدند. در غربالگری ثانویه سویه 24U با تولید mg/ml 892/0 تایلوزین و 28/1 برابر بیشتر از سویه وحشی به عنوان سویه مناسب انتخاب شد و در مرحله دوم تحت تیمار با EMS قرار گرفت. در نهایت سویه موتانتی با تولید mg/ml 18/2 تایلوزین و 12/3 برابر افزایش تولید نسبت به سویه وحشی بدست آمد. | ||
| عنوان مقاله [English] | ||
| Enhancement of tylosin production in Streptomyces fradiae by Mutation | ||
| نویسندگان [English] | ||
| D Zareh؛ M Azin؛ H Rasi؛ S.S Mirdamadi | ||
| Scientific Member of Biotechnology Institute | ||
| چکیده [English] | ||
| In order to increase tylosin production by Streptomyces fradiae, two mutagens, i. e. uv and EMS (Ethylmethanesulfonate) were used. Death curves were plotted for these mutagens. Optimum condition for obtaining mutants by uv radiation was determined by using a uv source lamp at a distance of 20 cm for 40-60 seconds. When EMS was used at 4% (w/v) concentration, the best mutants were isolated after 30 minutes of exposure to the mutagen. In the first mutation step, which was done by uv radiation, high tylosin producing strains were isolated by Plaque Agar method as the first screening step. For the second step of screening, a mutant strain (U24) with a production of 0.892 mg tylosin per ml was isolated, which was 1.27 times more productive than the wild type. The secondary mutation step on this mutant strain was performed by EMS. Finally, a mutant strain (UE1) with a production of 2.18 mg tylosin per ml was obtained, which was 3.12 times more productive than the wild type. | ||
| مراجع | ||
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1- Andrew, R. B., Neil, B., Eric. C., (1999) Impact of thioesterase activity on tylosin biosynthesis in Streptomyces fradiae. Chemistry and Biology. 6: 287-292##
2- Baltz, R. H. & Seno, E. T., (1981) Properties of Streptomyces fradiae mutants blocked in biosynthesis of the macrolide antibiotic tylosin. Antimicrobial Agent Chemotherapy. 20: 214-225##
3- Baltz, R. H., Seno, E. T., Stonesifer, J. & Wild, G. M., (1982)Biosynthesis of the macrolide antibiotic tylosin a preferred pathway from tylactone to tylosin. The Journal of Antibiotics. 36: 131-141##
4- Baltz, R. H., (1986) Mutagenesis in Streptomyces. In: Manual of Industrial Microbiology and Biotechnology. DeMain, A. and Solomon, N. eds., American Society for Microbiology. pp. 184-190##
5- British pharmacopoeia, (1993) London: HMSO, Appendix XIV, A 165-170##
6- Christopher, D. R., Shannon, L. W., Revill, W. P., Hideki, S., Matthew, M., Oleg, V. P., Saul, M., Hong, F., Steven, D. D. & Leonard. K., (2004) Production of hybrid 16-Membered macrolides by expressing combinations of polyketide synthase genes in engineered Streptomyces fradiae hosts. Chemistry and Biology. 11: 1465-1472##
7- Cruger, W. & Cruger, A., (1990) Antibiotics In: Biotechnology a Textbook of Industrial Microbiology. Sinauer Associates, Inc. pp. 256-258##
8- Cundliffe, E., Louise, A. & Mersone, D., (1993) Aspect of tylosin production and resistance in S. fradiae. In: Industrial Microorganisms: Basic and Applied Molecular Genetics. Baltz, R. H., Hegeman, G. D., and Skatrud, P. L. (eds.) Washington, D.C.: American Society for Microbiology, pp. 235-243 Academic Press.##
9- Cundliffe, E., Larg, S. L. & Gandecha, A. R., (1997) Analysis of four biosynthetic genes from the tyl LM region of the Streptomyces fradiae. Gene, 184: 197-203##
10- Du, B. C., Enoch. Y. P. & Mitsuyasu. O. (1998) Improvement of tylosin production from Streptomyces fradiae culture by decreasing the apparent viscosity in an Air-lift bioreactor. Journal of Fermentation and Bioengineering. 86: 413-417.##
11- Ikenda, H., Inoue. M. & Omura, S. (1983) Improvement of macrolide antibiotic producing streptomycete strains by the regeneration of protoplast. Journal of Antibiotics. 36: 283-288##
12- Lee, K. J. & Lee, H. S., (1991) Relationship between threonine dehydratase and biosynthesis of tylosin in Streptomyces fradiae. Journal of General Microbiology. 137: 2547-2553.##
13- Omura, S., Sadakane, N. & Matsubara, H., (1982) Bioconversion and biosynthesis of 16-membered macrolid antibiotic. Chemical Pharmacology Bulletin. 30: 223-229##
14- Omura, S., (1984) Macrolid antibiotics In: Chemistry, Biology and Practice. Academic press. pp. 1-35.##
15- Roberto, F., Marta, R., Encarnación, M., Bruno, D., José. L. B., (2000) Conjugation and transformation of Streptomyces species by tylosin resistance. FEMS Microbiology Letters, 186: 319-325## 16- Shazia, K,, Kalsoom, A, , Muhammad, A. G. Ruqia, I. Ahmad M. K. and Muhammad, M., Change in colony morphology and kinetics of tylosin production after UV and gamma irradiation mutagenesis of Streptomyces fradiae NRRL-2702. Microbiological Research (in press)##
17- Stephen, D., Pamela, F. C., Mingfu, L., Jacob. P., (2004) The tylosin-resistance methyltransferase RlmAII (TlrB) modifies the N-1 position of 23 S rRNA nucleotide G748. Journal of Molecular Biology, 337: 1073-1077##
18- Stephen, D., Lene, J., Satoko, Y., Dominique. F., (2008) Interaction of the tylosin-resistance methyltransferase RlmAII at its rRNA target differs from the orthologue RlmAI. Journal of Molecular Biology, 378: 969-975## | ||
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