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بررسی قدرت بیمارگری و ویژگی‌های مولکولی چند جدایه‌ی باکتری Bacillus thuringiensis روی کرم قوزه‌ی پنبه و شب‌پره‌ی پشت الماسی | ||
| مهار زیستی در گیاه‌پزشکی | ||
| مقاله 2، دوره 1، شماره 2، مهر 1392، صفحه 17-26 اصل مقاله (182.24 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/bcpp.2013.100606 | ||
| نویسندگان | ||
| مریم کلانتری* 1؛ رسول مرزبان1؛ زهرا معقولی فرد2؛ حبیب عباسی‌پور2 | ||
| 1بخش تحقیقات کنترل بیولوژیک، مؤسسه‌ی تحقیقات گیاه‌پزشکی | ||
| 2دانشکده‌ی کشاورزی دانشگاه شاهد | ||
| چکیده | ||
| باکتری Bacillus thuringiensis به‌خاطر مزیت‌‌های فراوان مانند اثر اختصاصی روی لارو حشرات آفت، عدم تأثیر نامطلوب روی محیط زیست و جاندارن غیر هدف و همچنین سهولت ترکیب و تلفیق با سایر روش‌‌های کنترل، جایگاه ویژه‌ای در مدیریت کنترل آفات دارد. اولین قدم به‌عنوان پایه و اساس تحقیق روی این باکتری، بررسی قدرت بیمارگری جدایهها و سویه‌‌های بیمارگر درکنترل آفات مورد هدف است. در این تحقیق قدرت بیمارگری سه جدایه‌ی بومی و یک نمونه‌ی تجاری(Dipel®) روی لارو سن دوم کرم قوزه‌ی پنبه،Helicoverpa armigera و شب‌پره‌ی پشت الماسی،Plutella xylostella در دمای 27 درجه‌ی سلسیوس و رطوبت نسبی 65% و دوره‌ی نوری 16:8 (تاریکی: روشنایی) بررسی شد. غلظت کشنده 50 درصد (LC50) سه جدایه‌ی بومی KD2، 20، 6R و نمونه‌ی تجاری دیپل روی لارو سن دوم کرم قوزه‌ی پنبه به‌ترتیب 107×21/1، 107×8/8، 107×39/1 و 107×91/6 اسپور در میلی‌لیتر محاسبه شد که بیمارگری جدایه‌یKD-2 ، با کمترین غلظت کشنده 50 درصد روی کرم قوزه‌ی پنبه بیشتر بود. غلظت کشنده‌ی 50 درصد جدایه‌های بومی فوق روی لاروهای سن دوم شب‌پره‌ی پشت الماسی به‌ترتیب 104×1/6، 103×4/3، 105×9/2 و 104×8/8 اسپور در میلی‌لیتر بود که بیشترین قدرت بیمارگری باکتری Bt مربوط به جدایه‌ی20 بود. در بررسی مولکولی جدایهها مشخص شد که جدایه‌های مورد بررسی دارای ژن‌های cry یکسانی نیستند. | ||
| کلیدواژه‌ها | ||
| Bacillus thuringiensis؛ کرم قوزه‌ی پنبه؛ شب‌پره‌ی پشت الماسی؛ قدرت بیمارگری؛ ویژگی مولکولی | ||
| عنوان مقاله [English] | ||
| Study of virulence and molecular characteristics of some Bacillus thuringiensis isolates on cotton bollworm and diamondback moth | ||
| نویسندگان [English] | ||
| Maryam Kalantari1؛ Rasoul Marzban1؛ Zahra Magollifard2؛ Habib Abbasipour2 | ||
| چکیده [English] | ||
| Bacillus thuringiensis bacteria because of many advantages such as specific effect on pest insects larvae, no adverse effects on the environment and non-target organisms and also easy combination with other methods of pest control, has an important role in pest management programs. The first step as a basis for doing research on this bacteria is to study virulence of different isolates and strains of the pathogen in the control of the target pest. In this research, the virulence of three native isolates and a commercial product (Dipel®) was evaluated on the larvae of cotton bollworm, Helicoverpa armigera and diamondback moth, Plutella xylostella at 27oC, a relative humidity of 65% and 16L:8D photoperiod. Based on the calculated results, 50% lethal concentration (LC50) of three native isolates of KD2, 20, 6R and commercial product of Dipel on the 2nd larval instars of H. armigera were obtained as 1.21×107, 8.8×107, 1.39×107 and 6.91×107 spore ml-1, respectively and KD2 isolate was the best strain among others. Results of 50% lethal concentration (LC50) bioassay of three native isolates and commercial sample of Dipel on the 2nd larval instars of P. xylostella were calculated as 6.1×104, 3.4×103, 2. 9×105 and 8.8×104 spore ml-1, respectively and isolate of 20 was the best strain among others. The molecular analysis of the isolates revealed that isolates cry genes are not identical. | ||
| کلیدواژه‌ها [English] | ||
| Bacillus thuringiensis, Helicoverpa armigera, Plutella xylostella, virulence, molecular characteristic | ||
| مراجع | ||
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