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کنترل بیولوژیک بیماری بلاست مرکبات با استفاده از چند گونه مخمر به‌دست آمده از باغات مرکبات شمال ایران | ||
| مهار زیستی در گیاه‌پزشکی | ||
| مقاله 5، دوره 1، شماره 1، شهریور 1392، صفحه 53-64 اصل مقاله (730.08 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22092/bcpp.2015.100599 | ||
| نویسندگان | ||
| فرید بیکی1؛ ابراهیم محمدی گل تپه1؛ حشمت اله رحیمیان* 2؛ مسعود شمس بخش1؛ علی برزگر2؛ آنتونی بوسکت3؛ جرج لالوکات3 | ||
| 1گروه بیماری‌‌‌شناسی گیاهی، دانشکده‌ی کشاورزی، دانشگاه تربیت مدرس، تهران | ||
| 2دانشکده‌ی کشاورزی، دانشگاه علوم کشاورزی و منابع طبیعی ساری | ||
| 3گروه میکروبیولوژی، دانشگاه UIB، اسپانیا | ||
| چکیده | ||
| بلاست مرکبات یکی از مهم‌ترین بیماری‌های مرکبات در استان‌های شمالی کشور می‌باشد که توسط چند گونه از باکتری بیمارگر سودوموناس (Pseudomonas spp.) ایجاد می‌شود. این بیماری در شرایط اقلیمی مساعد، خسارت‌های قابل توجهی را به باغات مرکبات وارد می‌سازد. در این بررسی، سعی شد تا از باغات مرکبات شمال کشور، مخمرهایی جداسازی و معرفی شوند که دارای توان کنترل بیولوژیک قابل قبولی علیه این بیمارگر باشند. ارزیابی در شرایط گلخانه‌با مایه‌زنی یک جدایه از باکتری بیماریزایPseudomonas syringae روی رقم نارنج صورت گرفت. پاشش مخمر سه بار و با فاصله هایزمانی دو روزه انجام شد و پس از آن، مایه‌زنی بیمارگر صورت گرفت. تجزیه‌و تحلیل آماری نتایج آزمایش براساس طرح بلوک‌های کامل تصادفی و مقایسه‌ی میانگین‌ها با استفاده از آزمون چند دامنه‌ی دانکن اجرا شد. برای شناسایی جدایه‌های برتر، ناحیه‌ی ITS آن‌ها با به‌کارگیری پرایمرهای ITS1 و ITS4 تکثیر و تعیین توالی شد. با مقایسه‌ی نتایج در پایگاه اینترنتی NCBI، مخمرهایSporobolomyces ruberrimus، Cryptococcus albidus، C. magnus و Rhodotorula sp.. به‌عنوان آنتاگونیست‌های برتر شناسایی شدند. براساس نتایج، S. ruberrimusمؤثرترین مخمر بوده و بیماری را بهتر از سایر گونه‌ها کنترل نمود. | ||
| کلیدواژه‌ها | ||
| کنترل بیولوژیک؛ بلاست مرکبات؛ Pseudomonas؛ Sporobolomyces؛ Cryptococcus و Rhodotorula | ||
| عنوان مقاله [English] | ||
| Biological control of citrus blast disease using some yeast strains isolated from citrus orchards in the northern provinces of Iran | ||
| نویسندگان [English] | ||
| Farid Beiki1؛ Ebrahim Mohamadi Gholtapeh1؛ Heshmatollah Rahimian2؛ Masood Shamsbakhsh1؛ Ali Barzegar2؛ Antony Busquets Bisbal3؛ Jorj Lalucat3 | ||
| چکیده [English] | ||
| Citrus blast caused by Pseudomonas spp. is one of the most important diseases in the northern citrus growing provinces of Iran which causes considerable losses to citrus orchards in conducive climatic conditions. In this study, we tried to isolate and introduce some yeast strains from citrus orchards with acceptable biological control potential against the disease. The evaluations were performed under green house conditions on sour orange seedlings. Yeast cell suspensions were sprayed three times with two-days intervals on seedlings before pathogen inoculation. Statistical analysis of the results was carried out using Randomized Complete Block experimental design and comparison of disease severity means was performed by Duncan's Multiple Range Test. For the identification of the effective yeast strains, ITS regions of their rRNA operon were amplified using universal primers (ITS1 and ITS4) by PCR and the amplified fragments were sequenced. Based on the above procedures and comparison of the sequences with those deposited in the GenBank, the effective yeasts were identified as Sporobolomyces ruberrimus, Cryptococcus albidus, C. magnus and Rhodotorula sp.According to the results, S. ruberrimus, was the most effective yeast and controlled the disease more efficiently than other species. | ||
| کلیدواژه‌ها [English] | ||
| biocontrol, Citrus blast, Pseudomonas, Sporobolomyces, Cryptococcus, Rhodotorula | ||
| مراجع | ||
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